Dr. Roberto Reif Profile

Dr. Roberto Reif

SPIE Involvement:

Author

Publications (13)

SPIE Journal Paper | 28 October 2014 Open Access

Platform to investigate aqueous outflow system structure and pressure-dependent motion using high-resolution spectral domain optical coherence tomography

Sepideh Hariri, Murray Johnstone, Yi Jiang, Steven Padilla, Zhehai Zhou, Roberto Reif, Ruikang Wang

JBO, Vol. 19, Issue 10, 106013, (October 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.10.106013

KEYWORDS: Optical coherence tomography, Image segmentation, Tissues, Adaptive optics, Mercury, Scanning electron microscopy, Imaging systems, Image resolution, Natural surfaces, Visualization

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 12 March 2014 Open Access

Improved microcirculation imaging of human skin in vivo using optical microangiography with a correlation mapping mask

Woo June Choi, Roberto Reif, Siavash Yousefi, Ruikang Wang

JBO, Vol. 19, Issue 03, 036010, (March 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.3.036010

KEYWORDS: Optical coherence tomography, Skin, Tissues, Tissue optics, In vivo imaging, Microcirculation imaging, Biomedical optics, Binary data, 3D image processing, Imaging systems

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 18 February 2014 Open Access

Functional evaluation of hemodynamic response during neural activation using optical microangiography integrated with dual-wavelength laser speckle imaging

Jia Qin, Lei Shi, Hequn Wang, Roberto Reif, Ruikang Wang

JBO, Vol. 19, Issue 02, 026013, (February 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.2.026013

KEYWORDS: Hemodynamics, Brain, Imaging systems, Laser speckle imaging, Blood circulation, Integrated optics, Integration, Neuroimaging, Speckle, System integration

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 28 October 2013 Open Access

Wide velocity range Doppler optical microangiography using optimized step-scanning protocol with phase variance mask

Lei Shi, Jia Qin, Roberto Reif, Ruikang Wang

JBO, Vol. 18, Issue 10, 106015, (October 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.10.106015

KEYWORDS: Scanners, Doppler effect, In vivo imaging, Imaging systems, Neuroimaging, Brain, Doppler tomography, Linear filtering, Tissues, Biomedical optics

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 20 March 2013 Paper

Phase-sensitive optical coherence tomography characterization of pulse-induced trabecular meshwork displacement in ex vivo non-human primate eyes

Peng Li, Roberto Reif, Zhongwei Zhi, Lin An, Elizabeth Martin, Tueng Shen, Murray Johnstone, Ruikang Wang

Proceedings Volume 8571, 85711S (2013) https://doi.org/10.1117/12.2006992

KEYWORDS: Optical coherence tomography, Tissues, Mercury, Eye, Tissue optics, Imaging systems, Veins, Resistance, Image segmentation, Visualization

Read Abstract +

Purpose. It is suspected that the abnormalities of aqueous outflow pump composed of trabecular meshwork (TM) and Schlemm’s canal (SC) results in the increased outflow resistance and then elevated intraocular pressure (IOP) in initial glaucoma. In order to explore the casual mechanism and the early diagnosis of glaucoma, the dynamic characterizations of aqueous outflow pump were explored.
Methods. As a functional extension of optical coherence tomography (OCT), tissue Doppler OCT (tissue-DOCT) method capable of measuring the slow tissue movement was developed. The tissue-DOCT imaging was conducted on the corneo-scleral limbus of 4 monkey eyes. The eye was mounted in an anterior segment holder, together with a perfusion system to control the mean IOP and to induce the cyclic IOP transients with amplitude 3 mm Hg at frequency 1 pulse/second. IOP was monitored on-line by a pressure transducer. Tissue-DOCT data and pressure data were recorded simultaneously. The IOP-transient induced Doppler velocity, displacement and strain rate of TM and the normalized area of SC were quantified at 7 different mean IOPs (5, 8, 10, 20, 30, 40, 50 mm Hg).
Results. The outflow system, including TM, SC and CCs, was visualized in the micro-structural imaging. The IOP-transient induced pulsatile TM movement and SC deformation were detected and quantified by tissue-DOCT. The TM movement was depth-dependent and the largest movement was located in the area closest to SC endothelium (SCE). Both the pulsations of TM and SC were found to be synchronous with the IOP pulse wave. At 8 mm Hg IOP, the global TM movement was around 0.65μm during one IOP transient. As IOP elevated, a gradual attenuation of TM movement and SC deformation was observed.
Conclusions. The observed pulsation of TM and SC induced by the pulsatile IOP transients was in good agreement with the predicated role of TM and SC acting as a biomechanical pump (pumping aqueous from anterior chamber into SC and from SC into CCs) in the aqueous outflow system. As the IOP elevated, the attenuated pulsation amplitude of the aqueous outflow pump indicated the failure of the mechanical pump and the increase of aqueous outflow resistance. The promising results revealed the potential of using the proposed tissue-DOCT for diagnosis and associated therapeutic guidance of the initial and progressive glaucoma process by monitoring the pulsation of the outflow pump.

SPIE Journal Paper | 1 October 2012 Open Access

Effects of hypoxia on cochlear blood flow in mice evaluated using Doppler optical microangiography

Suzan Dziennis, Roberto Reif, Zhongwei Zhi, Alfred Nuttall, Ruikang Wang

JBO, Vol. 17, Issue 10, 106003, (October 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.10.106003

KEYWORDS: Blood circulation, Hypoxia, Doppler effect, Optical coherence tomography, Blood vessels, Oxygen, Arteries, Blood, 3D image processing, Tissue optics

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 24 July 2012 Open Access

Phase-sensitive optical coherence tomography characterization of pulse-induced trabecular meshwork displacement in ex vivo nonhuman primate eyes

Peng Li, Roberto Reif, Zhongwei Zhi, Tueng Shen, Ruikang Wang, Elizabeth Martin, Murray Johnstone

JBO, Vol. 17, Issue 7, 076026, (July 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.7.076026

KEYWORDS: Optical coherence tomography, Tissues, Eye, In vivo imaging, Imaging systems, Adaptive optics, Image segmentation, Visualization, Eye models, Visual system

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 31 January 2012 Open Access

Multifunctional nanoprobe to enhance the utility of optical based imaging techniques

Yeongri Jung, Guangying Guan, Chen-Wei Wei, Roberto Reif, Xiaohu Gao, Matthew O'Donnell, Ruikang Wang

JBO, Vol. 17, Issue 1, 016015, (January 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.1.016015

KEYWORDS: Magnetism, Optical coherence tomography, Nanoparticles, Nanoprobes, Gold, Optical imaging, Absorption, Particles, Imaging systems, Scattering

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 30 January 2012 Paper

Depth profile absorber concentration reconstruction using photothermal optical coherence tomography

Roberto Reif, Guangying Guan, Ruikang Wang

Proceedings Volume 8213, 82132G (2012) https://doi.org/10.1117/12.910983

KEYWORDS: Optical coherence tomography, Solids, Temperature metrology, Refraction, Laser scattering, Scattering, Tissues, Tissue optics, Light scattering, Absorption

Read Abstract +

SPIE Journal Paper | 1 December 2011 Open Access

Depth profiling of photothermal compound concentrations using phase sensitive optical coherence tomography

Guangying Guan, Roberto Reif, Ruikang Wang, Zhihong Huang

JBO, Vol. 16, Issue 12, 126003, (December 2011) https://doi.org/10.1117/12.10.1117/1.3659211

KEYWORDS: Tissues, Optical coherence tomography, Solids, Profiling, Signal attenuation, Scattering, Natural surfaces, Temperature metrology, Refraction, Liquids

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 1 January 2008 Open Access

Analysis of changes in reflectance measurements on biological tissues subjected to different probe pressures

Roberto Reif, Mark Amorosino, Katherine Calabro, Ousama Aamar, Satish Singh, Irving Bigio

JBO, Vol. 13, Issue 01, 010502, (January 2008) https://doi.org/10.1117/12.10.1117/1.2870115

KEYWORDS: Tissues, Reflectivity, Blood, Scattering, Absorption, Blood vessels, Biological research, Natural surfaces, Optical properties, Fiber optics

Read Abstract +

DOWNLOAD PAPER

SPIE Journal Paper | 1 May 2007 Open Access

Optical method for real-time monitoring of drug concentrations facilitates the development of novel methods for drug delivery to brain tissue

Roberto Reif, Mei Wang, Shailendra Joshi, Ousama Aamar, Irving Bigio

JBO, Vol. 12, Issue 03, 034036, (May 2007) https://doi.org/10.1117/12.10.1117/1.2744025

KEYWORDS: Brain, Blood brain barrier, Tissue optics, Tissues, Blood, Animal model studies, Absorption, In vivo imaging, Optical fibers, Biomedical optics

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 15 February 2007 Paper

Absorption and scattering depth profile reconstruction in turbid media based on spectroscopy measurements

Roberto Reif, Irving Bigio

Proceedings Volume 6446, 644605 (2007) https://doi.org/10.1117/12.698030

KEYWORDS: Tissues, Scattering, Reflectivity, Absorption, Optical properties, Monte Carlo methods, Photons, Spectroscopy, Liquids, Natural surfaces

Read Abstract +

Showing 5 of 13 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years