Dr. Ron Gill Profile

Dr. Ron Gill

Assistant Professor at Univ Twente

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 24 March 2014 Paper

Toward efficient modification of large gold nanoparticles with DNA

R. Gill, K. Göeken, V. Subramaniam

Proceedings Volume 8955, 895504 (2014) https://doi.org/10.1117/12.2037239

KEYWORDS: Nanoparticles, Gold, Particles, Absorbance, Silver, Plasmons, Adsorption, Biomedical optics, Molecules, Surface plasmons

Read Abstract +

DNA-coated gold nanoparticles are one of the most researched nano-bio hybrid systems. Traditionally their synthesis has been a long and tedious process, involving slow salt addition and long incubation steps. This stems from the fact that both DNA and gold particles are negatively charged, therefore efficient interaction is possible only at high salt concentration. However, unmodified particles are susceptible to aggregation at high salt concentrations. Most of the recent modification methods involve the use of surfactants or other small molecules to stabilize the nanoparticles against aggregation, enabling faster modification. Here we present our result on an alternative route to reach fast modification in low salt conditions, namely, reduction of the charge of DNA. We will discuss both the use of natural DNA under acidic pH conditions, and the use of DNA with a cationic, spermine-based “tail” which is commercially available under the name ZNA. Additionally we introduce a characterization method based on ensemble localized surface plasmon resonance measurement (LSPR) which enabled us to extract the kinetics of DNA absorbance without the need for fluorescent tags. Lastly we show that the same ZNA-based modification protocol can be effectively used for silver nanoparticle modification.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years