Dr. Samarendra K. Mohanty Profile

Dr. Samarendra K. Mohanty

Chief Scientific Officer at Nanoscope Technologies LLC

SPIE Involvement:

Area of Expertise:

Biophotonics , Optical trapping , Laser scissors , Optical imaging , Photo-mediated therapy , Microscopy

Websites:

Company Website

Publications (72)

SPIE Conference Volume | May 11, 2018

Optogenetics and Optical Manipulation 2018

PROCEEDINGS ARTICLE | March 14, 2018

Optical stimulation and monitoring of the visual system using bioluminescent opsin (Conference Presentation)

Proc. SPIE. 10482, Optogenetics and Optical Manipulation 2018

KEYWORDS: Optical imaging, Modulation, Visualization, Visual system, Optogenetics, Bioluminescence, Retinal scanning, Visual cortex, Visual optics, Neurons

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PROCEEDINGS ARTICLE | March 14, 2018

In-vivo label-free optical detection of neural activities in retina (Conference Presentation)

Proc. SPIE. 10482, Optogenetics and Optical Manipulation 2018

KEYWORDS: Cornea, Visualization, Optical coherence tomography, Electrodes, Retina, In vivo imaging, Visual optics

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PROCEEDINGS ARTICLE | March 14, 2018

Targeted nano-enhanced Optical delivery of opsin for dry-AMD therapy (Conference Presentation)

Proc. SPIE. 10482, Optogenetics and Optical Manipulation 2018

KEYWORDS: Gold, Visualization, Retina, Molecules, Laser optics, Mouse models, Nanorods

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SPIE Journal Paper | August 18, 2017

Restoring vision in mice with retinal degeneration using multicharacteristic opsin

Wright, Weldon , Gajjeraman, Sivakumar , Batabyal, Subrata , Pradhan, Sanjay , Bhattacharya, Sulagna , Mahapatra, Vasu , Tripathy, Ashutosh , Mohanty, Samarendra

NPh Vol. 4 Issue 04

KEYWORDS: Retina, Visualization, Optogenetics, 3D modeling, Light emitting diodes, Therapeutics, Light, Neurophotonics, Luminescence, Eye

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SPIE Journal Paper | June 29, 2017

Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye

Batabyal, Subrata , Kim, Young-Tae , Mohanty, Samarendra

JBO Vol. 22 Issue 06

KEYWORDS: Molecules, Axons, Visualization, Ultrafast phenomena, Cones, Eye, Neurons, Molecular lasers, Tissues, Diffusion

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SPIE Conference Volume | April 28, 2017

Optogenetics and Optical Manipulation

PROCEEDINGS ARTICLE | April 19, 2017

Label-free optical detection of action potential in mammalian neurons (Conference Presentation)

Proc. SPIE. 10052, Optogenetics and Optical Manipulation

KEYWORDS: Gold, Signal to noise ratio, Luminescence, Phase interferometry, Neuroscience, Optogenetics, Action potentials, Electrophysiology, Singular optics, Neurons

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Electrophysiology techniques are the gold standard in neuroscience for studying functionality of a single neuron to a complex neuronal network. However, electrophysiology techniques are not flawless, they are invasive nature, procedures are cumbersome to implement with limited capability of being used as a high-throughput recording system. Also, long term studies of neuronal functionality with aid of electrophysiology is not feasible. Non-invasive stimulation and detection of neuronal electrical activity has been a long standing goal in neuroscience. Introduction of optogenetics has ushered in the era of non-invasive optical stimulation of neurons, which is revolutionizing neuroscience research. Optical detection of neuronal activity that is comparable to electro-physiology is still elusive. A number of optical techniques have been reported recording of neuronal electrical activity but none is capable of reliably measuring action potential spikes that is comparable to electro-physiology. Optical detection of action potential with voltage sensitive fluorescent reporters are potential alternatives to electrophysiology techniques. The heavily rely on secondary reporters, which are often toxic in nature with background fluorescence, with slow response and low SNR making them far from ideal. The detection of one shot (without averaging)-single action potential in a true label-free way has been elusive so far. In this report, we demonstrate the optical detection of single neuronal spike in a cultured mammalian neuronal network without using any exogenous labels. To the best of our knowledge, this is the first demonstration of label free optical detection of single action potentials in a mammalian neuronal network, which was achieved using a high-speed phase sensitive interferometer. We have carried out stimulation and inhibition of neuronal firing using Glutamate and Tetrodotoxin respectively to demonstrate the different outcome (stimulation and inhibition) revealed in optical signal. We hypothesize that the interrogating optical beam is modulated during neuronal firing by electro-motility driven membrane fluctuation in conjunction with electrical wave propagation in cellular system.

PROCEEDINGS ARTICLE | February 8, 2017

Multi-characteristic opsin enabled vision restoration

Proc. SPIE. 10052, Optogenetics and Optical Manipulation

KEYWORDS: Light sources, Eye, Light emitting diodes, Visualization, Retina, Luminescence, 3D modeling, Optogenetics, Therapeutics, Biological research, Light, Animal model studies

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SPIE Conference Volume | June 30, 2016

Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

PROCEEDINGS ARTICLE | May 19, 2016

Optical diagnosis of colon and cervical cancer by support vector machine

Proc. SPIE. 9887, Biophotonics: Photonic Solutions for Better Health Care V

KEYWORDS: Cancer, Tissues, Scattering, Scattering, Spectroscopy, Light scattering, Diagnostics, Optical spectroscopy, Fractal analysis, Colorectal cancer, Machine learning, Colon, Cervical cancer, In vivo imaging, Tissue optics, Algorithm development

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PROCEEDINGS ARTICLE | April 26, 2016

Digital holographic microscopy for imaging biophysical changes in cells during migration (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Near infrared, Holography, Microfluidics, Cancer, Digital holography, Modulation, Microscopy, Temporal resolution, System integration, Laser systems engineering

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PROCEEDINGS ARTICLE | April 26, 2016

Laser nano-surgery for neuronal manipulation (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Target detection, Proteins, Injuries, Nerve, Molecules, Ultrafast lasers, Optical simulations, Optical manipulation, Neurons, Axons

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PROCEEDINGS ARTICLE | April 26, 2016

Activation of cells using femtosecond laser beam (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Thermography, Near infrared, Visible radiation, Femtosecond phenomena, Laser therapeutics, Tissues, Telecommunications, Optogenetics, Tissue optics, Pulsed laser operation

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PROCEEDINGS ARTICLE | April 26, 2016

Label free detection of optogenetically stimulated cellular activity by low coherence interferometry (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Microscopes, Refractive index, Coherence (optics), Luminescence, Interferometry, Optogenetics, Spatial resolution, Phase measurement, Electrophysiology, System integration

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SPIE Conference Volume | April 3, 2015

Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II

SPIE Conference Volume | March 24, 2014

Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics

SPIE Conference Volume | March 13, 2013

Optogenetics: Optical Methods for Cellular Control

PROCEEDINGS ARTICLE | March 8, 2013

Optofluidic control of axonal guidance