A nanostructure which induces localized surface plasmon resonance (LSPR) can be utilized in visible light and near infrared (NIR) regions and it shows promising features as a bio-detector because LSPR state is changed easily by different bio-related materials. Owing to transparent property of many biomolecules as well as diluted states in base solutions, it is hard to distinguish each other by eye or microscope analysis. However, difference in molecular structure and composition makes difference in optical characteristics such as a refractive index or a dielectric constant. Therefore, our LSPR-based nanohole array structure which has high sensitivity to detect small changes in optical characteristics can be a great candidate for a bio detector. Here, we fabricated structural color filters (SCFs) to detect wavelength shifts for several biomolecules and optimized the nanohole array structures for high sensitivity. Periodic nanohole arrays were designed to present resonance peaks in visible light region for optical analysis and fabricated in Au or Al thin film layer. The spectral shifts were detected caused by biomolecules.
We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our
microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors.
Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To
induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right
channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth
day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is
doubled compared to the area on third day and increases by 20 times on ninth day.