We show the potential of Diffuse Reflectance Spectroscopy to identify Onchocerca Volvulus nodules, cysts, ganglions and lipomas on humans. We develop a new probe to take into account the geometry of the O. Volvulus model and implement a new measurement protocol and analysis method to deal with the melanin skin contribution. The nodule has a specific intensity and spectral signature compared to its background. It is therefore a relative measure that estimates a signal variation due to the worm and its environment. This may be used to monitor the worm state along time.
In the present work, we show the capacity of spatially resolved Diffuse Reflectance Spectroscopy (DRS) to identify live and dead worms in ex vivo Onchocerca ochengi nodules. It demonstrates the potential of DRS to diagnose the worm state and to monitor the effect of a drug on macrofilariae. These results have been obtained in constrained conditions, leading to the proposal of a rigorous acquisition and analysis protocol that may contribute to standardized measures in diffuse optics.