In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DM affects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. In this study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosis and increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2 . Non-irradiated cells were used as controls. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay), proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2- Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm2 and a fluence of 5 J/cm2 significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effects observed in this study are critical in the wound healing process.