We hypothesized that PEDF gene transduction in retina can provide single-dose treatment to prevent ganglion cell damage. Here, we present OCT guided ultrafast laser based non-viral targeted delivery PEDF-encoding genes to retina for neuroprotection. The ultrafast laser gene delivery showed layer-specific reliable expression of PEDF gene in retina without any detectable damage. Monitoring of IOP and electroretinogram after ultrafast laser transfection showed no adverse changes. The ultrafast laser transfection of large PEDF genes in retina exhibited significant therapeutic benefit in an injury model. Absence of any immune response in retina subsequent to ultrafast-laser transfection provides unique opportunity for repeated dosing.
We combine the real-time structural imaging capability of OCT with laser microirradiation for causing layer-specific dystrophies to mimic Retinitis Pigmentosa and dry-AMD model. In addition, we integrated an ERG-module for functional characterization after laser-injury to monitor the disease progression as well as to evaluate therapeutic efficacy. Here, we show creation of local atrophies with our combined OCT-Laser-ERG system in animal models and measurement of different cone and rod responses to focal stimulation light of different wavelengths. By varying the different mode of laser microirradiation and focal adjustment to the targeted depth, we demonstrate layer specific primary RPE injury.
Herein, we report use of near-infrared low-coherent light for non-contact, label-free in-vivo detection of retinal activity in response to visual illumination. Our multifractal phase-OCT employ phase/multifractal analysis to decipher layer specific cellular activity during visual stimulation to assess the functional state of retina. Our OCT-based interferometric technique coupled with in-depth multifractal analysis differentiated retinal activities between wild-type and mice with retinal dystrophy. Our findings open up possibility of clinical translation of multifractal phase-OCT for non-contact label free evaluation of retina health, progression of retinal dystrophies, and as well as for monitoring functional recovery after therapy.
Optical coherence tomography (OCT) is currently recognized as the gold standard for identifying retinal structural abnormalities in ophthalmology. However, its availability is often limited to large eye centers and research labs due to its high cost and lack of portability. We present a low-cost, portable spectral-domain OCT system with a total cost of materials under $6,000. Compared to current commercial systems, our design offers 50% size reduction and over 80% cost reduction. Image acquisition interface is incorporated and displayed onto a mounted 7-inch touchscreen. Human retinal imaging is demonstrated, and performance is compared with a commercial OCT system. Based on contrast-to-noise ratio analysis, the low-cost OCT demonstrates comparable imaging capabilities.