Ultrahigh resolution OCT (UHR OCT) was performed on normal and pathologic human skin biopsies using
an ultrabroad-bandwidth (260 nm) Titanium:sapphire laser, enabling sub-micrometer (0.9 μm) axial UHR OCT
resolution. Penetration, image contrast as well as resolution capabilities achieved are analyzed for optimum UHR OCT
in vivo performance. With the achieved resolution and the penetration depth, the transition between the dermis and the
epidermis is clearly visible by UHR OCT, and also the anomalies of pathologies, which have been confirmed
performing a comparison with histological analysis. Three dimensional UHR OCT of normal skin is demonstrated with
less than 3 μm axial resolution at video-rate with up to 50 B-scans/second, each tomogram consisting of 512×1024
pixels, resulting in 25 Megavoxels/second.