This study demonstrates improved kinetics for the formation of self-assembled monolayers (SAMs) of alkanethiols on
gold nanoparticle substrates. A computational model was developed to predict SAM growth kinetics. Based on the
predictions from the model, SAMs of 11-mercaptoundecanoic acid (11-MUA) and 1-octanethiol (1-OT) were formed by
incubation of gold nanoparticle chips in an ethanolic 10 mM solution within 20 min. The performance of this novel rapid
SAM formation protocol was compared with a conventional 24 hour incubation protocol. Binding capacity of the
alkanethiol SAM was investigated for a 20 min incubation protocol using biotin-streptavidin. For this purpose, the SAM
loaded gold nanoparticle chips were modified with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) to allow
attachment of EZ-Link amine PEG3 biotin to the 11-MUA molecules. Binding reactions were monitored in real time
using localized surface plasmon resonance (LSPR) spectroscopy. The resulting LSPR absorbance peak shift was
comparable to the experimental results for biotin-streptavidin reported in literature. Results of this study suggest that
formation of a high quality alkanethiol SAM within 20 min on gold nanoparticles surfaces is possible and could greatly
reduce the time and cost compared to conventional 24 h incubation protocols.
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