A micro-structured fiber-based system for identification and collection of fluorescent particles is demonstrated. An optical fiber probe with longitudinal holes in the cladding is used to retrieve fluorescent particles by exerting microfluidics forces. Laser induced fluorescent (LIF) is carried out by the fiber probe and an optical setup. When a particle with a previously chosen fluorescence wavelength is identified, a vacuum pump is activated collecting the particle into a hole. Green and red fluorescent polystyrene particles were detected and selectively retrieved.
We present a real-time method to measure the amplitude of thermal fluctuations in biological membranes by means of a new treatment of the defocusing microscopy (DM) optical technique. This approach was also applied to study the deformation of human erythrocytes to its echinocyte structure. This was carried out by making three-dimensional shape reconstructions of the cell and measuring the thermal fluctuations of its membrane, as the cell is exposed to the anti-inflammatory drug naproxen and as it recovers its original shape, when it is subsequently cleansed of the drug. The results showed biomechanical changes in the membrane even at low naproxen concentration (0.2 mM). Also, we found that when the cell recovered its original shape, the membrane properties were different compared to the nondrugged initial erythrocyte, indicating that the drug administration-recovery process is not completely reversible.