Fluorescent Light Energy (FLE) is a unique form of photobiomodulation that stimulates healing, reduces inflammation, and alleviates pain. The system works by exciting a chromophore in a topical substrate, which emits FLE with a broad spectral range (~400-700 nm) that is delivered to the target tissue below. Results from in vivo and in vitro studies have shown FLE modulates inflammation via down-regulation of pro-inflammatory cytokines such as IL-6 and TNF-α and stimulates mitochondria biogenesis1.
A recent study showed FLE-stimulated cells responded more potently compared to cells treated with light from an LED light source (“Mimicking Lamp”) designed to generate the same emission spectra and power intensity profile as FLE2. FLE-treated human dermal fibroblasts (HDF) experienced up-regulated collagen production, while a minor and nonsignificant effect was observed for the Mimicking Lamp-treated HDFs. These results suggest that photons generated by FLE either penetrate tissue differently or are absorbed differently compared to photons from a LED light source. Photonic properties of FLE that could impact tissue penetration or absorption may include polarity or coherency, leading to different cellular responses.
To investigate if light polarity may influence cellular responses to FLE stimulation, the present study applied linear and circular-polarizing filters to investigate the influence of FLE’s polarity on immune parameters. The data suggest that FLE polarity contributes to its impact on biological systems. Furthermore, the immunemodulatory impact of FLE was investgated in a pilot study on a human ex vivo skin model suggesting that central myeloid immune surface markes are modulated by FLE.
Research on photobiomodulation (PBM) has led to the development of various light-generating devices that can benefit a wide range of clinical indications. A novel approach of inducing PBM is through application of a Fluorescence Biomodulation (FB) System consisting of a blue light (peak wavelength between 440 and 460 nm) which activates topical photoconverter substrates containing specialized chromophores that generate fluorescence light energy (FLE). In clinical trials, FLE has been shown to modulate both healthy and disease-affected skin/soft tissue, providing a unique method for managing inflammatory skin conditions and accelerating healing. To better understand the biological impact of FB-induced FLE, we studied this system in vitro on dermal human fibroblasts (DHFs) and in vivo in canine deep pyoderma. In vitro data from stimulated DHFs exposed to an FB System showed a significant decrease in IL-6 production by 130.14% after 24 hr (p<0.001), compared to control groups. In canines with chronic deep pyoderma, the use of FB plus standard of care (SOC) treatment significantly reduced time to clinical resolution compared to controls that received SOC alone (p<0.001). Biopsies from lesional areas showed enhanced mitochondrial biogenesis in the FB lesions versus the SOC lesions, as supported by a significant increase in the number and size of mitochondria (89.31% and 90.15% respectively; p<0.0001). Significant modulation of inflammatory pathways, epithelialization, and angiogenesis were also demonstrated. These results support the use of FB Systems for skin conditions impacted by inflammation and offer a promising therapeutic solution to support its use in other medical conditions.