Extracellular Biosynthesis technique (EBS) for nanoparticles production has attracted a lot of attention as an
environmentally friendly and an inexpensive methodology. Our recent research was focused on the rapid approach of the
green synthesis method and the reduction of the homogeneous size distribution of nanoparticles using pulse laser
application. Noble nanoparticles (NNPs) were produced using various ethanol and water plant extracts. The plants were
chosen based on their biomedical applications. The plants we used were Magnolia grandiflora, Geranium, Aloe
‘tingtinkie’, Aloe barbadensis (Aloe Vera), Eucalyptus angophoroides, Sansevieria trifasciata, Impatiens scapiflora.
Water and ethanol extract, were used as reducing agents to produce the nanoparticles. The reaction process was
monitored using a UV-Visible spectroscopy. NNPs were characterized by Fourier Transfer Infrared Spectroscopy
(FTIR), Transmission Electron Microscopy (TEM), and the Dynamic Light Scattering technique (DLS). During the pulse
laser Nd-YAG illumination (λ=1064nm, 532nm, PE= 450mJ, 200mJ, 10 min) the blue shift of the surface plasmon
resonance absorption peak was observed from ~424nm to 403nm for silver NP; and from ~530nm to 520 nm for gold
NPs. In addition, NNPs solution after Nd-YAG illumination was characterized by the narrowing of the surface plasmon
absorption resonance band, which corresponds to monodispersed NNPS distribution. FTIR, TEM, DLS, Zeta potential
results demonstrated that NNPs were surrounded by biological molecules, which naturally stabilized nanosolutions for
months. Cytotoxicity investigation of biosynthesized NNPs is in progress.