In-situ Raman and fluorescence measurements were used to detect optically trapped single-walled carbon nanotubes (SWNTs). The in-situ fluorescence technique provides strong indirect visual evidence of optical trapping of SWNTs by monitoring the fluorescence quenching from a solution containing a mixture of carbon nanotubes and a fluorescent dye. The second monitoring technique uses in-situ Raman spectroscopy to show that in the presence of the optical trap, both the profile and the intensity of the nanotube Raman spectrum changes compared to when the optical trap is off. The Raman monitoring setup consists of two lasers which independently create the optical trapping path and Raman probing path. In this technique the Raman probe is capable of detecting structural information of the carbon nanotubes in the optical trap; therefore providing direct evidence of the local SWNTs concentration variation and chirality distribution. Both methods were used to verify optical trapping of SWNT and to determine the trapping threshold, trapping volume profile, and information on tube concentration change during optical trapping.