Ms. Shir Cohen Maslaton Profile

Shir Cohen Maslaton

at Tel Aviv University

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Proceedings Article | 21 June 2019 Paper

Wound healing assay of two competing cell types with dry mass measurement

Shir Cohen Maslaton, Natan Shaked

Proceedings Volume 11060, 110600Z (2019) https://doi.org/10.1117/12.2526841

KEYWORDS: Wound healing, Microscopes, Interferometry, Microscopy, Green fluorescent protein, Holograms, Phase interferometry, Refractive index, Imaging systems

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Wound healing assay is a method for evaluating of cell migration rate in vitro. In this assay, a scratch is performed in a confluent cell monolayer that usually contains only one type of cells. Interferometric phase microscopy (IPM) is a quantitative imaging technique, by which the optical path difference (OPD) of a sample is extracted. The OPD is equal to the thickness of the sample multiplied by its integral refractive index, and therefore provides us with quantitative information about the sample without labeling. Fluorescent probes are fluorescent compounds that can be attached to specific cells in order to provide ability of imaging the cells with specific contrast enhancement under fluorescence microscopy. Using a fluorescent microscope combined with an interferometric phase microscope, wound healing assay of a cell confluent monolayer that contains two types of cells is performed, while one type of cells is labeled with fluorescent probes for cell differentiation purposes. This allows us to check which cell type closes the wound faster, and to continually measure the dry mass of each cell population.

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