Mr. Shreesha Jagadeesh Profile

Shreesha Jagadeesh

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Proceedings Article | 4 March 2016 Presentation + Paper

Fluorescent detection of C-reactive protein using polyamide beads

Shreesha Jagadeesh, Lu Chen, Stewart Aitchison

Proceedings Volume 9715, 971505 (2016) https://doi.org/10.1117/12.2211373

KEYWORDS: Proteins, Plasma, Blood, Point-of-care devices, Bacteria, Magnetism, Microfluidic imaging, Microfluidics, Microscopes, Adsorption, Medicine, Coating, Statistical analysis

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Bacterial infection causes Sepsis which is one of the leading cause of mortality in hospitals. This infection can be quantified from blood plasma using C - reactive protein (CRP). A quick diagnosis at the patient’s location through Point-of- Care (POC) testing could give doctors the confidence to prescribe antibiotics. In this paper, the development and testing of a bead-based procedure for CRP quantification is described. The size of the beads enable them to be trapped in wells without the need for magnetic methods of immobilization. Large (1.5 mm diameter) Polyamide nylon beads were used as the substrate for capturing CRP from pure analyte samples. The beads captured CRP either directly through adsorption or indirectly by having specific capture antibodies on their surface. Both methods used fluorescent imaging techniques to quantify the protein. The amount of CRP needed to give a sufficient fluorescent signal through direct capture method was found suitable for identifying bacterial causes of infection. Similarly, viral infections could be quantified by the more sensitive indirect capture method. This bead-based assay can be potentially integrated as a disposable cartridge in a POC device due to its passive nature and the small quantities needed.

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