Histological examination has been the primary imaging modality in the diagnosis of eases such as cancer. However, since cells are three-dimensional in nature, the use of traditional nucleus to cytoplasm ratio (N/C) in two dimensions does not represent their three-dimensional structures. In this study, we used two-photon microscopy to acquired threedimensional images of normal human lung cell line Beas2B, human lung adenocarcinoma CL1-0 and CL1-5 cell lines. We determined N/C ratios in two- and three-dimensions and found that 2D N/C-ratio is more precise than 3D N/C-ratio in discriminating normal and cancer cells.
Hepatycotes in the liver may appear similar in morphology, however, heterogeneities may exist in cellular metabolism.
In this study, in vivo imaging of 6-carboxfluorescein diacetate (6-CFDA) metabolism in the liver was studied. We used
two-photon fluorescence microscopy and hepatic window to provide quantification in studying hepatocellular
metabolism. This model not only provides a potential platform for future study in hepatic responses and regulations, but
also contributes to the fine-tuning of organ-specific functions so as to open up a new era of exciting discoveries.