Prof. Shy Shoham Profile

Prof. Shy Shoham

Professor at NYU Langone Medical Ctr

SPIE Involvement:

Conference Program Committee | Author

Area of Expertise:

Neurophotonics , Digital Holography , Two photon microscopy

Websites:

Personal Website

Publications (17)

PROCEEDINGS ARTICLE | March 15, 2018

Real-time volumetric mapping of calcium activity in living mice by functional optoacoustic neuro-tomography (Conference Presentation)

Proc. SPIE. 10494, Photons Plus Ultrasound: Imaging and Sensing 2018

KEYWORDS: Optical sensors, Sensors, Calcium, Image sensors, Hemodynamics, In vivo imaging, Neuroimaging, Brain mapping, Neurons, Brain

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SPIE Journal Paper | March 15, 2018

Phase-controlled, speckle-free holographic projection with applications in precision optogenetics

Tal Aharoni, Shy Shoham

NPh Vol. 5 Issue 02

KEYWORDS: Holography, Optogenetics, Speckle, Holograms, Holography applications, Computer generated holography, Detection and tracking algorithms, Neurophotonics, Speckle pattern, Diffraction

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PROCEEDINGS ARTICLE | March 14, 2018

Volumetric optoacoustic mapping of fast neuronal activity in an isolated mouse brain model expressing genetically encoded calcium indicator (Conference Presentation)

Proc. SPIE. 10481, Neural Imaging and Sensing 2018

KEYWORDS: Image visualization, Visualization, Imaging systems, Calcium, Temporal resolution, Functional imaging, Spatial resolution, Neuroimaging, Brain mapping, Brain

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PROCEEDINGS ARTICLE | March 14, 2018

An all-optical system for rapid and deep interrogation of behaviorally relevant activity patterns (Conference Presentation)

Proc. SPIE. 10482, Optogenetics and Optical Manipulation 2018

KEYWORDS: Holography, Switching, Imaging systems, Image resolution, Wavefronts, Temporal resolution, Optogenetics, Pulsed laser operation, Neurons, Brain

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SPIE Journal Paper | July 12, 2017

Utah optrode array customization using stereotactic brain atlases and 3-D CAD modeling for optogenetic neocortical interrogation in small rodents and nonhuman primates

Ronald Boutte, Sam Merlin, Guy Yona, Brandon Griffiths, Alessandra Angelucci, Itamar Kahn, Shy Shoham, Steve Blair

NPh Vol. 4 Issue 04

KEYWORDS: 3D modeling, Computer aided design, Optogenetics, Brain, Solid modeling, 3D acquisition, Visual cortex, Visual process modeling, SolidWorks, Glasses

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PROCEEDINGS ARTICLE | April 24, 2017

In vivo optoacoustic monitoring of calcium activity in the brain (Conference Presentation)

Proc. SPIE. 10064, Photons Plus Ultrasound: Imaging and Sensing 2017

KEYWORDS: Visualization, Tissues, Scattering, Luminescence, Organisms, Calcium, Neuroscience, In vivo imaging, Neuroimaging, Brain

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Non-invasive observation of spatio-temporal neural activity of large neural populations distributed over the entire brain of complex organisms is a longstanding goal of neuroscience [1,2]. Recently, genetically encoded calcium indicators (GECIs) have revolutionized neuroimaging by enabling mapping the activity of entire neuronal populations in vivo [3]. Visualization of these powerful sensors with fluorescence microscopy has however been limited to superficial regions while deep brain areas have so far remained unreachable [4]. We have developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains [5]. The developed methodology can render 100 volumetric frames per second across scalable fields of view ranging between 50-1000 mm3 with respective spatial resolution of 35-150µm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically-encoded calcium indicator GCaMP5G demonstrated, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the depth barrier of optical imaging in scattering brains [6]. It was further possible to monitor calcium transients in a scattering brain of a living adult transgenic zebrafish expressing GCaMP5G calcium indicator [7]. Fast changes in optoacoustic traces associated to GCaMP5G activity were detectable in the presence of other strongly absorbing endogenous chromophores, such as hemoglobin. The results indicate that the optoacoustic signal traces generally follow the GCaMP5G fluorescence dynamics and further enable overcoming the longstanding optical-diffusion penetration barrier associated to scattering in biological tissues [6]. The new functional optoacoustic neuroimaging method can visualize neural activity at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques. Thus, in addition to the well-established capacity of optoacoustics to resolve vascular anatomy and multiple hemodynamic parameters deep in scattering tissues, the newly developed methodology offers unprecedented capabilities for functional whole brain observations of fast calcium dynamics.

PROCEEDINGS ARTICLE | April 27, 2016

Advanced multiphoton methods for in vitro and in vivo functional imaging of mouse retinal neurons (Conference Presentation)

Proc. SPIE. 9712, Multiphoton Microscopy in the Biomedical Sciences XVI

KEYWORDS: Optical design, Eye, Visualization, Retina, Functional imaging, Integrated optics, In vivo imaging, Retinal scanning, In vitro testing, Neurons

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PROCEEDINGS ARTICLE | April 26, 2016

Computational modeling of optogenetic neuronal excitation under complex illumination conditions using a Matlab-Neuron interface (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Optical design, Computational modeling, Interfaces, Neuroscience, Optogenetics, Tissue optics, Systems modeling, Dendrites, Neurons, Brain

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PROCEEDINGS ARTICLE | April 26, 2016

In vitro and in vivo analysis and characterization of engineered spinal neural implants (Conference Presentation)

Proc. SPIE. 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation

KEYWORDS: Injuries, Calcium, Image analysis, Image processing software, Optogenetics, In vivo imaging, Spinal cord, Clinical research, In vitro testing, Neurophotonics

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PROCEEDINGS ARTICLE | March 15, 2016

Estimation of the skull insertion loss using an optoacoustic point source

Proc. SPIE. 9708, Photons Plus Ultrasound: Imaging and Sensing 2016

KEYWORDS: Sensors, Signal attenuation, Ultrasonography, Microscopy, Ultrasonics, Bone, Transducers, Skull, Photoacoustic microscopy, Neuroimaging, Acoustics, Ferroelectric polymers, Brain imaging, Brain

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SPIE Journal Paper | July 23, 2015

All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation

Shir Paluch-Siegler, Tom Mayblum, Inbar Brosh, Inna Gefen, Shy Shoham, Hod Dana

NPh Vol. 2 Issue 03

KEYWORDS: Holography, Calcium, Neurons, Optogenetics, Imaging systems, Spatial light modulators, Objectives, Holograms, Neurophotonics, 3D image processing

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PROCEEDINGS ARTICLE | March 5, 2015

New insights and system designs for temporally focused multiphoton optogenetics

Proc. SPIE. 9329, Multiphoton Microscopy in the Biomedical Sciences XV

KEYWORDS: Mirrors, Tissues, Imaging systems, Scattering, Temporal resolution, Objectives, Optical resolution, Optogenetics, Tissue optics, Pulsed laser operation

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PROCEEDINGS ARTICLE | February 28, 2014

Two-photon in vivo imaging of retinal microstructures

Proc. SPIE. 8948, Multiphoton Microscopy in the Biomedical Sciences XIV

KEYWORDS: Two photon imaging, Eye, Imaging systems, Blood vessels, Retina, Luminescence, Objectives, Angiography, In vivo imaging, Retinal scanning

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SPIE Journal Paper | December 2, 2013

Special Section Guest Editorial: Optical Imaging, Sensing, and Light Interactions in Cells and Tissues

Natan Shaked, Shy Shoham, Bruce Tromberg, Israel Gannot

JBO Vol. 18 Issue 11

KEYWORDS: Biomedical optics, Optical sensing, Optical imaging, Tissues, Remote sensing, Confocal microscopy, Microscopy, Translational research, Optical coherence tomography, Neurophotonics

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PROCEEDINGS ARTICLE | March 4, 2013

Multimodal optoacoustic and multiphoton fluorescence microscopy

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Tissues, Particles, Luminescence, Image resolution, Transducers, Cavitation, Acoustics, Signal detection, Multiphoton fluorescence microscopy, Absorption

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PROCEEDINGS ARTICLE | February 22, 2013

Ultra-deep penetration of temporally-focused two-photon excitation

Proc. SPIE. 8588, Multiphoton Microscopy in the Biomedical Sciences XIII

KEYWORDS: Multiphoton microscopy, Biomedical optics, Scattering, Scattering media, Microscopy, Laser scattering, Objectives, Geometrical optics, Tissue optics, Diffraction gratings

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PROCEEDINGS ARTICLE | February 9, 2012

Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization

Proc. SPIE. 8226, Multiphoton Microscopy in the Biomedical Sciences XII

KEYWORDS: Microscopes, Multiphoton microscopy, Imaging systems, Cameras, Image processing, Luminescence, Geometrical optics, 3D image processing, Neurons, Diffraction gratings

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