In this experimental investigation, we suggest a new fluorescence microscope and method using a total internal reflection
fluorescence. Unlike the conventional a total internal reflection fluorescence scheme, it uses fluorescence obtained due to
the excitation due to the excitation of micro-objects occurring over the entire thickness of a sample, not a region in which
an evanescent field extends.
By the proposed scheme, the observation of the fluorescence of a biological micochip is possible without the installation
of optical filters on excitation and reception light paths. It is possible to observe a small amount of fluorescent dye due
to the reduction of the occurrence of optical noises and to simultaneously observe fluorescence images emitted from
various fluorescent dyes without optical filters, so that its construction become simple and the size decreases.