The present work describes the use of a recently established multiparametric methodology to study nanomaterial
toxicity. Using optimized methods, including proliferation-restricted cell types and endosomal buffer systems, the
effect of different types of nanomaterials on cultured cells were studied, focusing in particular on intracellular
particle degradation. Gold particles were quite resistant, whereas iron oxide degraded, with loss of magnetic
resonance contrast, but little toxicity associated. Quantum dots degraded more slowly, decreasing both fluorescence
quantum yield and cell viability over long-time periods. The multiparametric methodology is shown to be an
efficient screening strategy, allowing easy comparison of results obtained for different nanomaterials and hereby
helping to optimize nanoparticle design with improved safety.