The goal was to develop an upright microscope platform for the screening of slides employing one- and two-photon laser
scanning techniques. A highly compact, vibration damping unit was created, which combines novel concepts for moving
a slide in three dimensions, keeping it focused while doing so, scanning a laser-focus over the sample using novel
galvanometer-control concepts, combining and separating excitation and emission beam and spectrally dispersing the
emitted light by a linearized prism-spectrograph. Spectral detection is achieved by turning a 128 x 128 back-thinned EMCCD
detector in a continuously reading spectral point-detector. To make the unit even ore versatile, it can be turned into
a conventional wide field fluorescence microscope, enabling rapid routine observation to select regions of the sample for
a subsequent, more detailed confocal analysis.