Dr. Steve R. Hood Profile

Dr. Steve R. Hood

at GSK

SPIE Involvement:

Author

Publications (24)

Proceedings Article | 13 March 2024 Presentation

Presentation

Early selection of desired biopharmaceutical CHO cell lines using label-free multimodal optical microscopy and machine learning

Jindou Shi, Alexander Ho, Eric Chaney, Janet Sorrells, Kevin Tan, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume PC12846, PC1284603 (2024) https://doi.org/10.1117/12.3001298

KEYWORDS: Optical microscopy, Machine learning, Multiphoton fluorescence microscopy, Fluorescence lifetime imaging, Profiling, Ovary, Multiphoton microscopy, Multimodal imaging, Monte Carlo methods, Manufacturing

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Proceedings Article | 13 March 2024 Presentation

Presentation

Academia to industry translation of SLAM technology

Aneesh Alex, Kayvan Tehrani, Mantas Zurauskas, Matthew Durack, Reid Groseclose, Steve Hood, Stephen Boppart

Proceedings Volume PC12821, PC128210B (2024) https://doi.org/10.1117/12.3003121

KEYWORDS: Industry, Microscopy, Microscopes, Image analysis, Biological research, Autofluorescence, Analytical research, 3D modeling, Visualization, Visual process modeling

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Proceedings Article | 13 March 2024 Presentation

Presentation

Attention-based weakly-supervised deep learning for the identification and localization of drug fingerprints based on label-free hyperspectral CARS microscopy

Jindou Shi, Kajari Bera, Prabuddha Mukherjee, Aneesh Alex, Eric Chaney, Bradley Spencer-Dene, Jan Majer, Marina Marjanovic, Darold Spillman, Steve Hood, Stephen Boppart

Proceedings Volume PC12821, PC1282106 (2024) https://doi.org/10.1117/12.3001269

KEYWORDS: Microscopy, Deep learning, Biological samples, Nervous system, Complex systems, Vibration, Spatial learning, Profiling, Optical imaging, Nondestructive evaluation

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Proceedings Article | 13 March 2024 Presentation

Presentation

Predicting productivity in CHO cells based on co-occurrence of metabolism-related features in simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy

Alexander Ho, Jindou Shi, Eric Chaney, Janet Sorrells, Kevin Tan, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume PC12854, PC1285407 (2024) https://doi.org/10.1117/12.3003094

KEYWORDS: Microscopy, Fluorescence lifetime imaging, Autofluorescence, Feature extraction, Support vector machines, Second harmonic generation, Proteins, Ovary, Multimodal imaging, Monte Carlo methods

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Proceedings Article | 17 March 2023 Presentation

Presentation

Visualizing and quantifying cisplatin-induced nephrotoxicity using nonlinear optical imaging

Aneesh Alex, Eric Chaney, Crysthiane Ishiy, Ciarán Fisher, Darold Spillman, Marina Marjanovic, Jan Roger, Reid Groseclose, Steve Hood, Stephen Boppart

Proceedings Volume 12357, 1235705 (2023) https://doi.org/10.1117/12.2649232

KEYWORDS: Visualization, Optical imaging, Tissue optics, Luminescence, Harmonic generation, Second-harmonic generation, Multiphoton microscopy, Microscopy, Medicine, Kidney

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Proceedings Article | 15 March 2023 Presentation

Presentation

Applications of Raman microscopy in cancer biology and drug discovery (Conference Presentation)

Valerie Brunton, Martin Lee, Ander Maguregui, Craig Steven, Manasa Ravindra, Romain Enjalbert, Michaela Noskova, Steve Hood, Alison Hulme, Miguel Bernabeu

Proceedings Volume PC12392, PC123920Y (2023) https://doi.org/10.1117/12.2652435

KEYWORDS: Raman spectroscopy, Tumors, Microscopy, Cancer, Drug discovery, Biology, Visualization, Tissues, Spectroscopy, Resistance

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Proceedings Article | 15 March 2023 Presentation

Presentation

Characterization of Chinese hamster ovary (CHO) cell lines by simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for upstream bioprocessing (Conference Presentation)

Alexander Ho, Jindou Shi, Eric Chaney, Corey Snyder, Aneesh Alex, Remben Talaban, Janet Sorrells, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume PC12383, PC1238302 (2023) https://doi.org/10.1117/12.2646378

KEYWORDS: Microscopy, Ovary, Image processing, Luminescence, Image segmentation, Third-harmonic generation, Second-harmonic generation, Proteins, Multimodal imaging, Manufacturing

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Proceedings Article | 6 March 2023 Presentation

Presentation

Machine learning-assisted label-free multimodal optical bioimaging for biopharmaceutical CHO cell line characterization

Jindou Shi, Alexander Ho, Corey Snyder, Eric Chaney, Janet Sorrells, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume PC12371, PC123710D (2023) https://doi.org/10.1117/12.2648499

KEYWORDS: Microscopy, Image segmentation, Fluorescence lifetime imaging, Feature extraction, Biomedical optics, Therapeutic antibodies, Proteins, Profiling, Ovary, Neural networks

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Proceedings Article | 7 March 2022 Presentation

Presentation

Applying label free imaging to GSK drug discovery projects: current successes and future opportunities

Steve Hood, Aneesh Alex

Proceedings Volume PC11938, PC1193802 (2022) https://doi.org/10.1117/12.2606230

KEYWORDS: Drug development, Drug discovery, Tissue optics, Raman spectroscopy, Positron emission tomography, Optical tracking, Nonlinear optics, Network security, Monoclonal antibody therapy, Monoclonal antibodies

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Proceedings Article | 7 March 2022 Presentation

Presentation

Differential uptake of antisense oligonucleotides in mouse hepatocytes and macrophages revealed by simultaneous 2P-fluorescence and coherent Raman imaging

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Kajari Bera, Chi Zhang, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendall Frazier, Steve Hood, Stephen Boppart

Proceedings Volume PC11938, PC119380D (2022) https://doi.org/10.1117/12.2608740

KEYWORDS: Coherence imaging, Luminescence, Raman spectroscopy, Liver, Receptors, Two photon imaging, Tissues, Multimodal imaging, Molecules, Modulation

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Proceedings Article | 7 March 2022 Presentation

Presentation

Label free and non-destructive imaging approach for cell line selection of genetically modified functional Chinese Hamster Ovary (CHO) Cells

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Eric Chaney, Remben Talaban, Maire Daly, Darold Spillman, Marina Marjanovic, Gary Finka, Steve Hood, Stephen Boppart

Proceedings Volume PC11964, PC119640C (2022) https://doi.org/10.1117/12.2608764

KEYWORDS: Ovary, Nondestructive evaluation, Fluorescence lifetime imaging, Optical imaging, Mode conditioning cables, Microscopy, Luminescence, Image analysis, Genetics, Cell biology

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Proceedings Article | 7 March 2022 Presentation

Presentation

Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging

Kajari Bera, Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Darold Spillman, Marina Marjanovic, Ahmed Shabana, Russell Johnson, Steve Hood, Stephen Boppart

Proceedings Volume PC11938, PC119380A (2022) https://doi.org/10.1117/12.2609087

KEYWORDS: Multiphoton microscopy, Microscopy, Nanoparticles, Raman spectroscopy, Green fluorescent protein, CARS tomography, Multiphoton fluorescence microscopy, Luminescence, Proteins, Multimodal imaging

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Proceedings Article | 7 March 2022 Presentation

Presentation

Nanodiamond based phantom for assessing multimodal fluorescence and multiharmonic imaging systems

Mantas Žurauskas, Aneesh Alex, Jaena Park, Steve Hood, Stephen Boppart

Proceedings Volume PC11951, PC1195103 (2022) https://doi.org/10.1117/12.2608258

KEYWORDS: Imaging systems, Luminescence, Microscopy, Control systems, Calibration, Microscopes, Harmonic generation, Scattering, Quality systems, Optical instrument design

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Proceedings Article | 2 March 2022 Presentation

Presentation

Label-free multimodal multiphoton imaging of Liver-on-a-Chip models

Aneesh Alex, Terrence Roh, BanuPriya Sridharan, Jindou Shi, Prabuddha Mukherjee, Eric Chaney, James Tunstead, Jan Majer, Kajari Bera, Darold Spillman, Marina Marjanovic, Jason Ekert, Steve Hood, Stephen Boppart

Proceedings Volume PC11972, PC1197202 (2022) https://doi.org/10.1117/12.2608861

KEYWORDS: Liver, Multiphoton microscopy, Multimodal imaging, 3D modeling, Multiphoton fluorescence microscopy, Microscopy, Optical imaging, Fluorescence lifetime imaging, Toxicology, Tissues

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Proceedings Article | 5 March 2021 Presentation

Presentation

Single-cell analysis pipeline for quantification of intracellular drug distribution by CARS/2PF/FLIM

Jindou Shi, Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume 11649, 1164911 (2021) https://doi.org/10.1117/12.2577041

KEYWORDS: Image analysis, Fluorescence lifetime imaging, Optical imaging, Microscopy, Luminescence, CARS tomography

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Proceedings Article | 5 March 2021 Presentation

Presentation

FlimTK: a Python-based toolkit package for fluorescence lifetime imaging microscopy (FLIM) data analysis

Jindou Shi, Mantas Žurauskas, Prabuddha Mukherjee, Aneesh Alex, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume 11649, 1164907 (2021) https://doi.org/10.1117/12.2577939

KEYWORDS: Fluorescence lifetime imaging, Microscopy, Data analysis, Image analysis, Visualization, Visual analytics, Molecular biology, Luminescence, Image visualization, Graphics processing units

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Proceedings Article | 5 March 2021 Presentation

Presentation

Label-Free detection of antisense oligonucleotide uptake in Murine cells using hyperspectral CARS imaging

Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Derek Shi, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendal Frazier, Steve Hood, Stephen Boppart

Proceedings Volume 11656, 116561F (2021) https://doi.org/10.1117/12.2578061

KEYWORDS: CARS tomography, Hyperspectral imaging, Microscopy, Raman spectroscopy, Proteins, Picosecond phenomena, Molecules, Luminescence, In vivo imaging, Imaging spectroscopy

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Proceedings Article | 5 March 2021 Presentation

Presentation

Enhancing liver on a chip Complex In Vitro Model studies using label-free optical imaging

Aneesh Alex, BanuPriya Sridharan, Edita Aksamitiene, Chi Zhang, Jindou Shi, Prabuddha Mukherjee, Mantas Zurauskas, Darold Spillman, Marina Marjanovic, Jason Ekert, Steve Hood, Stephen Boppart

Proceedings Volume 11624, 116240G (2021) https://doi.org/10.1117/12.2578531

KEYWORDS: Optical imaging, In vitro testing, Animal model studies, Multiphoton microscopy, Multiphoton fluorescence microscopy, Microscopy, 3D modeling, Tissues, Fluorescence lifetime imaging, Tumors

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Proceedings Article | 5 March 2021 Presentation

Presentation

Differential uptake of antisense oligonucleotides in mouse hepatocytes and macrophages revealed by simultaneous 2P-fluorescence and CARS

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Derek Shi, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendal Frazier, Steve Hood, Stephen Boppart

Proceedings Volume 11624, 116240E (2021) https://doi.org/10.1117/12.2578037

KEYWORDS: Two photon imaging, Receptors, Luminescence, Liver, Tissues, Multimodal imaging, Molecules, Modulation, Chemistry, CARS tomography

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Proceedings Article | 10 March 2020 Presentation

Presentation

Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling (Conference Presentation)

Conor Horgan, Anika Nagelkerke, Thomas Whittaker, Valeria Nele, Lucia Massi, Ulrike Kauscher, Jelle Penders, Mads Bergholt, Steve Hood, Molly Stevens

Proceedings Volume 11251, 112511G (2020) https://doi.org/10.1117/12.2541025

KEYWORDS: Raman spectroscopy, In vitro testing, Molecular imaging, Molecular spectroscopy, 3D image processing, Oxides

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Proceedings Article | 4 March 2019 Paper

Paper

Optimisation of multimodal coherent anti-Stokes Raman scattering microscopy for the detection of isotope-labelled molecules

Dale Boorman, Iestyn Pope, Wolfgang Langbein, Steve Hood, Paola Borri, Peter Watson

Proceedings Volume 10890, 1089005 (2019) https://doi.org/10.1117/12.2509280

KEYWORDS: Molecules, Raman spectroscopy, Chemical analysis, CARS tomography, Microscopy, Microscopes, Hyperspectral imaging, Chemical species, Data analysis, Luminescence

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Proceedings Article | 4 March 2019 Presentation

Presentation

Label-free multimodal multiphoton microscopy for monitoring of cell and tissue preservation and degradation in ex vivo whole organs (Conference Presentation)

Jang Hyuk Lee, Aneesh Alex, Jose Rico-Jimenez, Eric Chaney, Steve Hood, Stephen Boppart

Proceedings Volume 10881, 108810D (2019) https://doi.org/10.1117/12.2507482

KEYWORDS: Tissues, Multiphoton microscopy, Fluorescence lifetime imaging, Optical properties, Ultrafast imaging, Nonlinear optics, Light-matter interactions, Signal generators, Cell mechanics, Image processing

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Proceedings Article | 4 March 2019 Presentation

Presentation

Drug toxicity assessment using label-free multimodal nonlinear optical imaging (Conference Presentation)

Aneesh Alex, Jang Hyuk Lee, Jose Rico-Jimenez, Eric Chaney, Sunish Mohanan, Stephen Boppart, Steve Hood

Proceedings Volume 10859, 108590I (2019) https://doi.org/10.1117/12.2510032

KEYWORDS: Toxicity, Multimodal imaging, Optical imaging, Multiphoton microscopy, Multiphoton fluorescence microscopy, Microscopy, Fluorescence lifetime imaging, Toxicology, Drug development, Drug discovery

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Proceedings Article | 4 March 2019 Presentation

Presentation

Exploring the applications of imaging in pharmaceutical R&D (Conference Presentation)

Steve Hood

Proceedings Volume 10859, 1085903 (2019) https://doi.org/10.1117/12.2511273

KEYWORDS: Molecules, Tissues, Proteins, Drug discovery, Geography, Mathematics, Chemistry, Chemical analysis, Mass spectrometry, Radio optics

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Showing 5 of 24 publications

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