We report the fabrication and validation of a microfluidic chip for fluorescence detection, which incorporates in the same glass substrate the microfluidic network, the excitation, the filtering, and the collection elements. The device is fabricated in a hybrid approach combining different technologies, such as femtosecond laser micromachining and RF sputtering, to increase their individual capabilities. The validation of the chip demonstrates a good wavelength selective light filtering and a limit of detection of a 600-nM concentration of Oxazine 720 perchlorate dye.
We demonstrate a way of light harvesting in integrated microfluidic chips fabricated by femtosecond laser micromachining. The architecture consists of waveguide arrays fabricated in the vicinity of the microchannel filled with a fluorescent organic solution (e.g., polyfluorene solution). Amplified spontaneous emission from the microchannel is efficiently coupled by the waveguides to the outside of the chip.
We report the fabrication of micro-Fresnel lenses by femtosecond laser surface ablation on one-dimensional (1-D) polymer photonic crystals. This device is designed to focus the transmitted wavelength (520 nm) of the photonic crystal and filter the wavelengths corresponding to the photonic band-gap region (centered at 630 nm, ranging from 530 to 700 nm). Integration of such devices in a wavelength selective light harvesting and filtering microchip is envisaged.