The dynamical characteristics of integral mechanical properties of drying droplets of blood serum, urine and saliva were studied by measuring the acoustic-mechanical impedance (AMI) using a computer-controlled setup described earlier. The method is based on the following idea. When a 5μl liquid drop is drying on the surface of a quartz resonator plate oscillating at a constant frequency (equal to the resonance frequency of an unloaded resonator, 60 kHz), there arises a shear wave highly sensitive to the formation and growth of a new phase at the liquid-quartz interface. The experimental setup measures a change in the complex electric conductivity of the liquid-quartz system, calculates the parameters of the AMI dynamics in the drying drop, and displays their variation on the monitor at the real time scale in the form of a curve. At a time the optical The properties of drying drops were observed. For each disease or a physiological state the geometrical features of the curves were extracted and then the shape indices were calculated. In the cases under study each "pathology" group differed from the "norm" by its specific shape index, by which diseases of other nature did not differ. Possible mechanisms behind formation of the morphological and dynamical differences in drying drops of biological liquids of healthy and sick people are discussed.
The effect of XeCl laser radiation on carbonic anhydrase solution is studied. It is investigated that kinetics of protein aggregation is strongly influenced by both laser fluence and repetition rate. The theoretical model is constructed which allows one to explain qualitatively the features of experimental findings.
We propose a method for studying multi-component liquids based on recording of the dynamics of the acoustic-mechanical impedance (AMI) of a drop that dries up on the surface of a quartz resonator oscillating with ultrasound frequency. The magnitude of the AMI is an integral characteristic of the physical properties of the drop including its viscosity, composition, surface tension, moistening, and inner structure. Using liquids of different types as the example, it is shown that each liquid possesses its individual 'portrait', determined by the character of the phase transitions. In the authers’ opinion, this method can be used for the screening identification of liquids (determining the degree of consistency with the standards) in solving a number of scientific and practical problems, as well as in biology, chemistry, food and drug examination and medicine. Unique scopes of this method in medical diagnostics, vine, food and drug identification and determination of inner structure of solutions are demonstrated.
Experimental and clinical studies carried out with the help of optical, biochemical, potentiometric and EPR-methods showed that blood reaction to light irradiation is similar to that of nonequilibrium medium characterized by oscillating and multistationary regimes that makes it impossible to a priori predict the only possible reaction. The mechanism of biological effect of low-intensity light is realized through deviations in oxidation-reduction blood system balance due to selective activation of these or those key components which include, in particular, compounds containing Fe and Cu ions. Value and sign of these reactions are determined by the state of this system at each time moment and are nontrivially associated with the light of different wavelength ranges. A possibility to predict the reaction of the patient to phototherapy based on the results of preliminary irradiation of his blood in vitro is shown. The technique and laboratory criteria making it possible to predict directivity and efficiency of the patient's reaction to future-to-be irradiation (irrespective of the manner of irradiation) and to choose the required wavelength range providing for the optimal therapeutic effect at this particular time moment is worked out.