We give a broad discussion of existing typical works devoted to particle image processing. We propose the approach based on the postprocessing of coherent images of the particles at various planes of the volume. These images can be obtained both by reconstruction of inline digital hologram and by means of defocussing of the lens with high numerical aperture. Processing of the reconstructed holograms or recorded images is carried out using the proposed image analysis approach based on the edge-point linking and thresholding technique, which is considered to be simple to implement and reliable. After the review of existing methods and approaches, we noted that, in general, only cases of low concentrations are considered and, therefore, we investigated the performance of our proposed approach for characterization of particles of high density in a volume of optical medium. In this study of the method, we increase the concentration of particles until we ensure that every volume element comprises many particle images, yet these images do not create a speckle pattern, and look for the concentrations at which normalized density distributions of the particles can be constructed with an acceptable error for us. It is shown that the proposed approach exhibits good results of recognition and allows investigation of high concentrations.
The study of particles that are transparent to the probing radiation but introduce a phase delay appears to be relevant especially in the fields of biology and medicine. In this paper, we propose an approach to the study of the distribution of transparent particles suspended in a volume of optical medium, which combines the method of digital holography and the concept of singular optics. For the numerical study of the particles, we use a method, based on the obtainment and analysis of zerograms that correspond to the spatial distributions of amplitude zeros of the complex amplitude of the field. We explore the features of the application of analysis of the amplitude zero distributions in solving the problem of studying transparent particles suspended in a volume of the optical medium. We investigate the effect of various particle parameters on the recorded hologram and the structure and distribution of the amplitude zeros of the electromagnetic field. We demonstrate the use of histograms of distributions of local densities of amplitude zeros for the characterization of a higher number of transparent particles in volume. Numerical experiments on the use of analysis of the amplitude zeros of the field have shown that there are a number of particular qualities in the distribution of the zeros of amplitude, which can be subsequently used to develop more accurate and efficient method of characterization of transparent particles.
In this paper, the problems of recognition of individual particle images in the volume of optical medium conditioned upon their coherent superposition are described. To evaluate the efficiency of methods of particle image recognition, a typical problem of analysis of the intensity distributions formed by laser radiation scattered on suspended particles in a volume of an optical medium is considered. As a result of applying of the method of statistical accounting of particle images, based on the edge-point linking and thresholding technique, normalized density distributions of particles in the image plane are obtained. Evaluation of the performance of applied recognition method for individual particle images is conducted using correlation analysis to assess the quality of obtained images.