In this study, Optical Coherence Tomography (OCT) was used to investigate the effect of short term ozone exposure
on both front and back surfaces of the leaves of <i>Allium tuberosum</i> plant. Plant leaves were subjected to treat with
240ppb level of ozone, and imaging data were acquired from back and front surfaces of the leaves. Variations of the
biological activations were monitored based on the biospeckles. Standard deviations (SD) of OCT temporal signal at
each point were calculated and used to visualize the effect of ozone exposure. Leaf back surface showed higher SDs in
biospeckle OCT signal in comparison to these of the front surface. These observations prove that the short term
ozone stress on plant can be investigated successively with biospeckle OCT imaging technique.