We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in
order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering
technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the
microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope
to simultaneously monitor in real time both the cell membrane and in-depth phenomena.
In this paper, we discuss the possibility of making a super-axially-resolved image of a biological sample using
supercritical angle diffusion. This labeling-free approach is suitable to any microscope equipped with a NAobj <
1.33 microscope objective and can be used either for conventional intensity imaging or for quantitative phase
imaging. We expose some results on beads an cells showing the potential of this method.
Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer
resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent
marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an
alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively
filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system
and its combination with other imaging techniques.