Microfluidic connectors play a key role in the microfluidic packaging systems. This paper presents the results of a development of a new design of polymeric surface connector for single channel connection between tube and microfluidic chip. The design was evaluated using finite element analysis, which takes into account assembly forces and stresses on components. The design offers an easy and simple connection process and is compatible with most polymeric microfluidic systems using different diameter tubes. Prototype PMMA and polycarbonate connectors have been produced for 1.6 mm diameter PEEK (polyethylethylene ketone) tube. These connectors can be connected and disconnected many times and will be suitable for many cases with special requirements. A series of performance tests, which include leakage, connection and disconnection force test, and reliability, have been conducted and to demonstrate the functionality of sealing and reached excess 1.37 MPa (200 psi.) without leakage and pulling force reached 40 N (9 lbf).