The lateral shearing phase shifting interference microscopy with low coherent illumination for tomography of living cells has been proposed. To create projections of a sample at different angles the angular scanning of the probe beam was used. The experimental results of erythrocyte tomogram reconstruction are presented.
Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells are represented. The immersion 100x, N.A. equals 1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.
To analyze the optical tomography reconstruction (OTR) methods applications for reconstruction of meso- and large-scale irregularities of minor gas and aerosol components in the atmosphere and near Earth space (NES) the principal model problems of OTR are considered. The results of computer simulation to reconstruct the model irregular structure in NES are presented and discussed.
The paper presents some evaluations of possibility to use the optical tomographics methods for investigation of natural and artificial irregularities in the near circumterrestrial space. The results obtained during research of artificial irregularities and polar airglow are described.
The iteration algorithm of three-dimensional tomography, the modified Gershberg-Papulis algorithm is developed. Its accuracy characteristics have been determined by numerical simulations. Much attention has been paid to the peculiarities of preliminary processing of the projection images from physical experiment. Algorithm is used for the reconstruction of the local emission coefficients in a micropinch discharge plasma. The analysis has revealed admissible accuracy of the algorithm, its sufficient stability to measurements noises, and the applicability in the processing of a real experiment.