Near-infrared (NIR) fluorescence has the potential to provide surgeons with real-time intraoperative image-guidance.
Increasing the signal-to-background ratio of fluorescent agents involves delivering a controllable excitation
fluence rate of proper wavelength and/or using complementary imaging techniques such as FLIM. In this study we
describe a low-cost linear driver circuit capable of driving Light Emitting Diodes (LEDs) from DC to 35 MHz, at high
power, and which permit fluorescence CW and lifetime measurements. The electronic circuit Gerber files described in
this article and the list of components are available online at www.frangionilab.org.