Proceedings Article | 9 February 2012
KEYWORDS: FT-IR spectroscopy, Nervous system, Transmittance, Neodymium, YAG lasers, Absorption, Nd:YAG lasers, Proteins, Laser irradiation, Tissue optics
Fourier Transform Infrared Spectroscopy (FTIR) is a very sensitive tool which is capable of providing
strong insight on structural and functional changes in lipids and proteins induced by laser radiation.
In the present work cockroach nervous tissue and chitin from tibia region are irradiated with Nd: YAG
laser (λ= 1064 nm, Power =150mW) via fiber optics (Numerical aperture=0.22, diameter = 8 μ). Nd: YAG laser
exposure time is varied from 10 sec to 50 sec for nervous tissue and chitin. FTIR (Fourier Transform Infra Red
spectra) of cockroach nervous tissue and chitin are compared before and after laser irradiation. The FTIR spectrum
of non irradiated cockroach nervous tissue shows clearly the peaks due to O-H (Carboxylic acid), C=O (Amide I),
C=C (Aromatic), N=0 (Nitro), C-H (Alkenes), CH (Aromatics). FTIR Spectra of non irradiated cockroach chitin
clearly shows O-H (Carboxylic acid), C=O (Carbonyl stretch), C=C (Aromatic), N=O (Nitro), C-O, (anhydrides),
C-H (Alkenes stretch) group.
FTIR spectra of laser radiated nervous tissue from cockroach tibia and chitin shows significant changes in
transmittance for O-H, C=O, C=C, C-H, N=O, C-O and C-H groups. The percentage transmittance increases for
O-H, C=C group for exposure time 10sec, 40sec and 50 sec for nervous tissue. The percentage transmittance
increases for O-H, C=C group for exposure time 10sec, 20sec, 30sec and 40 sec for chitin. The study shows
clearly that FTIR spectroscopy of nervous tissue can reveal the interactions between infrared laser light and
nervous tissue.
