Indocyanine green (ICG) is a near-infrared fluorescence contrast agent, which has enormous potential in early tumor diagnosis and therapy. The objective of this study is to develop biodegradable nanoparticles entrapping ICG and to characterize its intracellular uptake and photodynamic activity in different cancer cell lines. Nanoparticles entrapping ICG were engineered, characterized and the intracellular uptake of ICG was investigated in B16-F10 and C-33A cancer cell lines. The photodynamic activity of ICG-loaded nanoparticles was also investigated. The nanoparticles enhanced the intracellular uptake of ICG and showed significant photodynamic activity, especially at very low ICG concentrations. These preliminary studies indicate the potential of efficient tumor cell delivery and tumoricidal effect of ICG when incorporated in nanoparticles.
Degradation of Indocyanine green (ICG) in aqueous media, limits its application in early tumor diagnosis and therapy. Thus, the objective of this study is to develop biodegradable nanoparticles entrapping ICG and to establish its effectiveness in providing overall stability to ICG. Nanoparticles entrapping ICG were engineered and characterized. The degradation kinetics of ICG in the nanoparticles was investigated in aqueous media. The degradation of ICG in aqueous nanoparticle suspension followed first-order kinetics. Nanoparticles enhanced aqueous, photo and thermal-stability of ICG.
The objective of this study is to engineer a novel nanoparticlulate system for use in early tumor diagnosis. Indocyanine green (ICG)-loaded biodegradable nanoparticles were prepared by using biodegradable polymer, poly(DL-lactic-co-glycolic acid) (PLGA). The ICG entrapment, nanoparticle size, shape, zeta potential the release of ICG from nanoparticles was determined. Also, the effect of ICG entrapment on fluorescence spectra of ICG was measured. The engineered nanoparticles were nearly spherical in shape and efficiently entrapped ICG. The release profile of the nanoparticles was exponential. The entrapment of ICG in nanoparticles caused reduction in its peak fluorescence intensity and shifted its wavelength of peak fluorescence to higher values.