Prof. Watt W. Webb Profile

Prof. Watt W. Webb

Deceased at Cornell Univ

SPIE Involvement:

Author

Publications (17)

Proceedings Article | 13 March 2013 Paper

High resolution, large field-of-view endomicroscope with optical zoom capability

Dimitre Ouzounov, David Rivera, Watt Webb, Chris Xu

Proceedings Volume 8575, 85750G (2013) https://doi.org/10.1117/12.2003107

KEYWORDS: Zoom lenses, Tissues, Image resolution, Objectives, Tissue optics, Endoscopes, Optical components, Lung, Polymer optical fibers, Scanners

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SPIE Journal Paper | 9 April 2012 Open Access

In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope

Christopher Brown, David Rivera, Dimitre Ouzounov, Watt Webb, Chris Xu, Ina Pavlova, Wendy Williams, Sunish Mohanan

JBO, Vol. 17, Issue 04, 040505, (April 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.4.040505

KEYWORDS: Tissues, In vivo imaging, Kidney, Colon, Liver, Imaging systems, Infrared imaging, Luminescence, Second-harmonic generation, Endoscopes

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SPIE Journal Paper | 5 April 2012 Open Access

Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung

Ina Pavlova, Watt Webb, Kelly Hume, Stephanie Yazinski, Teresa Southard, Robert Weiss, James Flanders

JBO, Vol. 17, Issue 03, 036014, (April 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.3.036014

KEYWORDS: Lung, Luminescence, Tumors, Tissues, Imaging spectroscopy, Multiphoton microscopy, Lung cancer, Inflammation, Diagnostics, Visualization

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Proceedings Article | 23 February 2011 Paper

Epifluorescence light collection for multiphoton microscopic endoscopy

Christopher Brown, David Rivera, Chris Xu, Watt Webb

Proceedings Volume 7893, 78930A (2011) https://doi.org/10.1117/12.875475

KEYWORDS: Scattering, Light scattering, Optical fibers, Tissue optics, Tissues, Endoscopy, Bladder, Natural surfaces, In vivo imaging, Objectives

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Proceedings Article | 26 February 2010 Paper

Multiphoton microscopy as a diagnostic imaging modality for lung cancer

Ina Pavlova, Kelly Hume, Stephanie Yazinski, Rachel Peters, Robert Weiss, Watt Webb

Proceedings Volume 7569, 756918 (2010) https://doi.org/10.1117/12.841017

KEYWORDS: Lung, Luminescence, Tissues, Lung cancer, Second-harmonic generation, Tissue optics, Multiphoton microscopy, Diagnostics, Tumor growth modeling, In vivo imaging

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Proceedings Article | 23 February 2009 Paper

Human bladder cancer diagnosis using multiphoton microscopy

Sushmita Mukherjee, James Wysock, Casey Ng, Mohammed Akhtar, Sven Perner, Ming-Ming Lee, Mark Rubin, Frederick Maxfield, Watt Webb, Douglas Scherr

Proceedings Volume 7161, 716117 (2009) https://doi.org/10.1117/12.808314

KEYWORDS: Tumors, Bladder cancer, Biopsy, Bladder, Cystoscopy, Second-harmonic generation, Endoscopy, Diagnostics, Harmonic generation, Tissues

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Proceedings Article | 28 April 2005 Paper

Dark fraction and blinking of water-soluble quantum dots in solution

Jie Yao, Daniel Larson, Warren Zipfel, Watt Webb

Proceedings Volume 5705, (2005) https://doi.org/10.1117/12.606131

KEYWORDS: Luminescence, Quantum dots, Biological research, Fluorescence spectroscopy, Spectroscopy, Fluorescence correlation spectroscopy, Molecules, Pathology, Pollution control, Quantum efficiency

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Proceedings Article | 21 June 2004 Paper

In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael Levene, Daniel Dombeck, Rebecca Williams, Jesse Skoch, Gregory Hickey, Karl Kasischke, Raymond Molloy, Martin Ingelsson, Edward Stern, Jochen Klucken, Brian Bacskai, Warren Zipfel, Bradley Hyman, Watt Webb

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.537285

KEYWORDS: GRIN lenses, Multiphoton microscopy, In vivo imaging, Tissues, Ovary, Brain, Luminescence, Alzheimer's disease, Pathology, Lenses

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Proceedings Article | 1 November 2002 Paper

Multiphoton fluorescence microscopy in biology

Ahmed Heikal, Watt Webb

Proceedings Volume 4812, (2002) https://doi.org/10.1117/12.451382

KEYWORDS: Luminescence, Multiphoton fluorescence microscopy, Molecules, Microscopy, Absorption, Green fluorescent protein, Mode conditioning cables, Quantum efficiency, Fluorescence correlation spectroscopy, Spectroscopy

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Proceedings Article | 24 April 2001 Paper

Chronic imaging of amyloid plaques in the live mouse brain using multiphoton microscopy

Brian Bacskai, Stephen Kajdasz, R. Christie, Warren Zipfel, Rebecca Williams, Karl Kasischke, Watt Webb, B. Hyman

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424546

KEYWORDS: Brain, Neuroimaging, In vivo imaging, Multiphoton microscopy, Skull, Tissues, Proteins, Luminescence, Angiography, Blood vessels

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Proceedings Article | 13 November 1996 Paper

Multiphoton fluorescence excitation: new spectral windows for biological imaging

Chris Xu, Jerome Mertz, J. Shear, Watt Webb

Proceedings Volume 2819, (1996) https://doi.org/10.1117/12.258073

KEYWORDS: Luminescence, Microscopy, Multiphoton fluorescence microscopy, Photons, Multiphoton microscopy, Ultraviolet radiation, Confocal microscopy, Absorption, Biological research, Proteins

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Proceedings Article | 3 September 1993 Paper

Force and membrane compliance measurements using laser interferometry and optical trapping

Luke Ghislain, Watt Webb

Proceedings Volume 1889, (1993) https://doi.org/10.1117/12.155726

KEYWORDS: Particles, Optical tweezers, Laser interferometry, Calibration, Atomic force microscopy, Sensors, Biomedical optics, Laser beam diagnostics, Bacteria, Yeast

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Proceedings Article | 13 August 1992 Paper

Signal measurements of multilayer refractive write-once data storage media

James Strickler, Watt Webb

Proceedings Volume 1663, (1992) https://doi.org/10.1117/12.137569

KEYWORDS: Data storage, Multilayers, Optical storage, Scattering, Digital image correlation, Refractive index, Laser scattering, Head, Image processing, Microscopes

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Proceedings Article | 1 June 1992 Paper

Two-photon lithography for microelectronic application

En-Shinn Wu, James Strickler, W. Harrell, Watt Webb

Proceedings Volume 1674, (1992) https://doi.org/10.1117/12.130367

KEYWORDS: Absorption, Microelectronics, Optical lithography, Lithography, Photoresist materials, Microscopes, Mode locking, Photoresist developing, Pulsed laser operation, Scanning electron microscopy

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Proceedings Article | 1 April 1992 Paper

Time-resolved fluorescence imaging and background rejection by two-photon excitation in laser-scanning microscopy

David Piston, David Sandison, Watt Webb

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58230

KEYWORDS: Luminescence, Microscopy, Photons, Confocal microscopy, Modulation, Microscopes, Laser spectroscopy, Biochemistry, Phase shift keying, Ultraviolet radiation

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A new dimension in quantitative fluorescence microscopy may be accessed by imaging of fluorescence decay times. To obtain spatially resolved information from microscopic sample locations, one must not only have sufficient optical resolution and detection sensitivity but also the ability to exclude fluorescence photons originating from outside the focal volume of interest. This background rejection is measured by the signal-to-background ratio, which must be large if three-dimensional information is to be obtained from a thick fluorescence sample. Two-photon excitation in laser scanning microscopy has an unparalleled ability to meet these demands. The two-photon excitation of a transition normally in the ultraviolet arises from the simultaneous non-linear absorption of two red photons. Because two-photon excitation depends on the square of the incident intensity, the resulting fluorescence is limited to the focal volume where the photon density of the focused laser illumination is high. This localization limits photobleaching and any photodamage to the focal plane of the image. This property is a major advantage over widefield or confocal microscopy. Two-photon excitation provides the depth discrimination associated with confocal microscopy without a confocal spatial filter, an advantage which allows for major simplifications of the apparatus. The resolution and background rejection properties of two-photon excitation have been calculated and measured, and have been shown to be identical to an ideal confocal microscope with the same optical wavelengths. Combined, these properties provide the ideal conditions for time-resolved imaging of fluorescence decay dynamics in order to characterize the submicroscopic environment of the fluorophore molecules within the specimen. A preliminary apparatus was designed and built to test these concepts, and it was found that fluorescence decay time images of living cells can be conveniently recorded with diffraction limited resolution in a few seconds of image acquisition time.

Proceedings Article | 1 February 1992 Paper

Two-photon excitation in scanning laser microscopy (Abstract Only)

James Strickler, Watt Webb

Proceedings Volume 1556, (1992) https://doi.org/10.1117/12.134895

KEYWORDS: Confocal microscopy, Microscopy, Chromophores, 3D metrology, Luminescence, Ultraviolet radiation, Objectives, Lenses, CCD image sensors, Sensors

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