The 488nm laser is the most important excitation light source of flow cytometry. The indirect diode (frequency-doubled diode) 488nm lasers are used in the excitation of Becton Dickinson (BD) AccuriTM C6. For using cost effective lasers, we have validated direct diode 488nm lasers as the replacement component of frequency-doubled diode laser. BD Bioscience issued the protocols to cover wavelength, power, noise, and polarization at the operation temperature range of cytometer. Pavilion Integration Corporation (PIC) tested 6 samples as the component validation of direct diode 488nm lasers based on the protocols from BD Biosciences. BD Bioscience also tested one of laser samples to further validate the test results of power, noise, and polarization from PIC.