The fluorescents detector sub-system is one of most important sub-systems of fluorescence-activated cell sorting (FACS) flow cytometry. The Photomultiplier Tube (PMT) detector sub-system are used in the fluorescent detection of Becton Dickinson (BD) AccuriTM C6 Plus. For using cost effective solid-state detectors (SSD) sub-system, we have validated SSD sub-system as the replacement sub-system of PMT. BD Bioscience and Pavilion Integration Corporation (PIC) use BD Instruments (Accuri C6 Plus) as test tools to test the SSD of PIC vs. PMT. We further ran the Swap test and Repeatability as well as ran test at the operational temperature range of cytometer. We also ran the Linearity test. All results were passed. Based on these results, BD Bioscience teams will run the biological sample testing to cover the test of CFSE- Proliferation Assay, Cell Cycle Analysis, and Apoptosis.
The 488nm laser is the most important excitation light source of flow cytometry. The indirect diode (frequency-doubled diode) 488nm lasers are used in the excitation of Becton Dickinson (BD) Accuri<sup>TM</sup> C6. For using cost effective lasers, we have validated direct diode 488nm lasers as the replacement component of frequency-doubled diode laser. BD Bioscience issued the protocols to cover wavelength, power, noise, and polarization at the operation temperature range of cytometer. Pavilion Integration Corporation (PIC) tested 6 samples as the component validation of direct diode 488nm lasers based on the protocols from BD Biosciences. BD Bioscience also tested one of laser samples to further validate the test results of power, noise, and polarization from PIC.