Mast cell (MCs) researches have received worldwide attention and achieved great achievements. Degranulation of MCs is not only related to anaphylaxis, but also plays an important role in the formation and progression of tumor. The existing detection methods could not fully reflect the degree of cell degranulation. In this paper, surface-enhanced Raman scattering (SERS) was used to detect and analyze the degranulation degree of MCs treated with different concentrations of C48/80 (compound 48/80, a mast cell activator). The culture supernatants of cells treated with different concentrations of C48/80 (0 μg/mL, 2 μg/mL and 10 μg/mL) were mixed with Ag colloids and high quality SERS spectra were acquired. The assignment of SERS bands combined with differential spectra analysis indicated that biomolecules associated with cell degranulation in the C48/80 treated groups were changed compared with the control group, including a decrease in the percentage of lipid content and an increase in the relative contents of collegen and phosphatidylserine. Furthermore, principal component analysis (PCA) and linear discriminant analysis (LDA) diagnostic algorithms were employed to analyze and distinguish the SERS spectra of different cell degranulation groups with high sensitivity, specificity and accuracy. The larger value of the integration area under the ROC curve also suggested the greater forecast accuracy. This exploratory work demonstrates that the combination of SERS technology and PCA-LDA algorithm has great potential for developing a label-free, comprehensive and accurate method for detecting cell degranulation.
Renal artery occlusion is an unavoidable process during operations such as nephrectomy, carcinoma resection, kidney transplantation and so on. For minimizing the kidney injury, the optimal renal ischemia time was experimentally investigated. Optical coherence tomography (OCT) is a high resolution imaging modality can non-invasively image biotissue in vivo and in real time. In order to study the relationship between tubular injury and the renal ischemia time, OCT images of Wistar rat kidneys endured various ischemia time were obtained throughout the whole ischemia-reperfusion procedure. The tubular density and the average diameter of uriniferous tubules were considered as biomarker of kidney activity, and the quantitative results well described the degree of kidney injury.
Optical coherence tomography (OCT) is a useful optical biopsy tool. Its potential in the evaluation of living kidney has been demonstrated. One of such applications is to predict the acute tubular necrosis (ATN) associated with kidney transplantation. The light dense and lucent regions seen in 2D OCT scanning are considered as a useful marker of the renal tubules. In this study, the OCT examination of living human kidney was carried out using a swept source (SS) OCT (SS-OCT) system. The light lucent regions in the cortex obtained on the OCT scan were defined as low signal cavities. The structure features of characteristic cavities in 2D and simulated 3D OCT images were quantitatively analyzed using Amira and Matlab programs. Although the imaging acquisition and real-time analysis were feasible for the examination of donor kidney before and after the transplantation, as the imaging acquisition was obtained under the hand-hold fashion, OCT images might become blurred and the tubules became hardly distinguishable from cortex background, especially for 3D images. In order to optimize the scanning parameters of the OCT imaging process, the influence of the jittering of the living kidney on the quality of OCT imaging and the distortion of the renal tubule structure were studied.
Optical coherence tomography (OCT) is an imaging technology which can be used to obtain the high resolution cross sectional image of living biological tissues. It has been used to evaluate the structure and function of animal and human kidneys. Preliminary animal and human data suggest that OCT imaging might be a useful non-invasive tool for characterizing renal tubular lumens, such as the opening status of tubular lumens. In this pilot study, living animal kidneys (dog, rat and mouse) were imaged using a swept source OCT (SS OCT) or spectral domain OCT(SD OCT). In vivo imaging scans were carried out using an OCT microscope setup (5×) and by placing the imaging probe above the surface of the living kidney. Semi-quantitative analysis of the OCT images was performed to evaluate the density of the kidney tubules on the surface layer of the cortex. In addition, histological images of the kidneys were restructured to form nephron three-dimensional structure for comparison with the 3D OCT imaging. This study suggests that quantitative OCT imaging might be useful for visualizing the fine structure of the living kidney and determining the density of renal tubules.
Human hair is a keratinous tissue composed mostly of flexible keratin, which can form a complex architecture consisting of distinct compartments or units (e.g. hair bulb, inner root sheath, shaft). Variations in hair shaft morphology can reflect ethnical diversity, but may also indicate internal diseases, nutritional deficiency, or hair and scalp disorders. Hair shaft abnormalities in cross section and diameter, as well as ultramorphological characterization and follicle shapes, might be visualized non-invasively by high-speed 2D and 3D optical coherence tomography (OCT). In this study, swept source OCT (ThorLabs) was used to examine human hair. Preliminary results showed that the high-speed OCT was a suitable and promising tool for non-invasive analysis of hair conditions.
Near infrared Nd:YAG pulsed laser treatment had been proved to be an efficient method to treat large-sized vascular
malformations like leg telangiectasia for deep penetrating depth into skin and uniform light distribution in vessel.
However, optimal clinical outcome was achieved by various laser irradiation parameters and the key factor governing
the treatment efficacy was still unclear. A mathematical model in combination with Monte Carlo algorithm and finite
difference method was developed to estimate the light distribution, temperature profile and thermal damage in epidermis,
dermis and vessel during and after 1064 nm pulsed Nd:YAG laser irradiation. Simulation results showed that epidermal
protection could be achieved during 1064 nm Nd:YAG pulsed laser irradiation in conjunction with cryogen spray
cooling. However, optimal vessel closure and blood coagulation depend on a compromise between laser spot size and
Nonablative skin remodeling is a new light treatment approach for photodamaged skin. Compared to ablative CO<sub>2</sub> or
Er:YAG laser resurfacing, dermabrasion, and chemical peels, the clinical objective of nonablative skin remodeling is to
maximize thermal damage to upper dermis while minimizing injury to the epidermis and surrounding tissue,
consequently decreasing potential complications and shortening long recuperation periods.
Histological analysis of preoperative and postoperative biopsies using H&E or special stains has indicated the dermal
thermal injury, which resulting in collagen denaturation, is the most important mechanism of nonablative skin
remodeling for improving skin situation. And the extent of improvement of skin situation corresponded to the formation
of a new band of dense, compact collagen bundles in the papillary dermis. The diversity of individual skin condition
influences the choice of pulsed light treatment parameters, and further influences the degree of dermal thermal damage,
thus the efficacy of nonablative skin remodeling remains unstable.
Recently, multiphoton microscopy has show a promising application for monitoring skin thermal damage, because
collagen could produce strong second harmonic generation (SHG). And SHG intensity is presumably proportional to the
percentage of collagen in dermis. In this paper, the auto-fluorescence (AF) intensity and SHG intensity of mice skin
irradiated by pulsed Nd:YAG laser were measured and imaged with multiphoton microscope, and the results show the
ratio of SHG to AF decreases with the increase of irradiation exposure dose, and could be a quantitative technique to
assess dermal thermal damage, and could further benefit the choice of light treatment parameters.
With low risk of complications and no down-time, the non-ablative photorejuvenation is playing an increasing role
in the therapy of the photodamaged skin. The light dose is one of the key factors that affect the performance of the
photorejuvenation. Monitoring the tissue response during the procedure of laser irradiation would help to determine
whether the light dose is appropriate. In this study, we developed a new approach to monitor the instant response of
tissue irradiated by laser device by measuring the change of the total attenuation coefficient of the tissue with optical
coherence tomography. The total attenuation coefficient was deduced from the raw data obtained by OCT with single
scattering mode. Spatial and temporal equalizations were employed to improve the signal-to-noise ratio. We measured in
vivo the total attenuation coefficients of the mouse back skin before and after laser irradiation. The total attenuation
coefficients of the tissue reduced approximately 60% immediately after laser irradiation and then kept constant in a short
time. The reduction of the attenuation coefficient depended on the light dose. These results demonstrated that the new
approach could be a potential tool for monitoring in clinic in the future.
Intense pulsed light system in combination with contact cooling is a useful tool for treating photodamaged skin. It can
emit broadband light, and target different kind of pigment in skin. But the pressure on skin often influences the
temperature field distribution inside the skin when the treatment head contacts skin. In this paper, we assumed the
pressure on skin would cause the changes of thermal diffusivity and optical absorption coefficient. Under these
assumptions, the temperatures inside skin induced by intense pulsed light system with contact cooling were modeled
using Monte Carlo method and explicit finite difference bioheat conduction equation. The results suggest that pressure
imposed on skin surface should be taken into account when using pulsed light system with contact cooling to treat
lesions inside the skin.
Several methods have been used to improve the esthetic appearance of photodamaged skin including dermabrasion, chemical peels and laser resurfacing using CO<sup>2</sup> and Er:YAG laser. These procedures sacrifice epidermis, resulting in a long recuperation period and potential complications including persistent scarring, infection, hyperpigmentation, etc. Compared to ablative CO<sup>2</sup> or Er:YAG laser resurfacing, non-ablative photorejuvenation technologies are playing an increasing role in the treatment of photodamaged skin. The clinical objective of which is to maximize thermal damage to upper dermis while minimizing injury to overlying skin. A variety of laser and non-laser systems have been used in the initial stage for this treatment. In our review, different treatment modalities have resulted in varying degrees of clinical effects. The basic mechanisms relate to improvement in employing non-ablative technologies are also discussed. Photorejuvenation is still not a fully established clinical tool for cosmetic treatment according to our review, therefore more research on basic mechanisms should be made.
“Nonablative skin photorejuvenation” is a new treatment for photoaged skin, which is to wound the upper dermis in order to induce dermal fibrosis and improve the clinical appearance. In this paper, the penetration depths of the light emitted by intense pulsed light source (IPLS) were analyzed, and the results show the IPLS was suitable for nonablative skin photorejuvenation. A new method with thermal texture maps (TTM) technology for exploring the mechanisms and repairing processes in the nonablative treatment of photoaged skin was presented.