The interaction between drugs and serum albumin is the theoretical basis of pharmacology research. Kangai injection with invigorating Qi, enhancing the immune function, is widely used for a variety of malignant tumor treatment. Fluorescence spectroscopy was adopted due to its high sensitivity and other advantages. The interaction between kangai injection and human serum albumin (HSA) in physiological buffer (pH 7.4) was investigated by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results of fluorescence spectrum at three temperature (296K, 303K and 310K) showed the degree of binding at 310K is the highest. Also, the maximum emission peak has a slight blue shift, which indicates that the interaction between kangai injection and HSA has an effect on the conformation of HSA. That is, the microenvironment of tryptophan increase hydrophobic due to the increase of the concentration of kangai injection. Results obtained from analysis of fluorescence spectrum and fluorescence intensity indicated that kangai injection has a strong ability to quench the intrinsic fluorescence of HSA. And according to the Stern-Volume equation, the quenching mechanism is static quenching, which is further proved by the UV-Vis absorption spectroscopy.
Optical diagnostic technique, due to its rapid and non-invasive for the diagnosis diseases at the cellular level, can be performed in vivo and allow for real-time diagnosis. While light scattering method is capable of characterizing the structural properties of tissue at the cellular and subcellular scale. In this paper, the spherical models of cells light scattering were established based on Mie, and the distribution curves of scattering intensity in the range of 0~180 degrees were got to explore change rule of cells light scattering information at the molecular level. Also, a platform for experiments used to measure the light scattering information of cells was built to get the change rule of cells light scattering information in wide angular range. And the particle size distribution (PSD) of cells was got by the inversion algorithm. A comparative analysis between numerical simulation and goniometric measurements revealed that the forward-scattering and side-scattering were influenced by the particle size of cells and relative index of refraction between cells and surrounding media. It could also be concluded that it was necessary to get and analyze the light scattering information of larger scattering angle range, which may be related to the intracellular organelles and nucleus.
As with the number of cancer increases year by year, so it is important to be found and treated earlier. With biological cells and tissues are sensitive to infrared and visible light, cell morphology and physical structure of the optical properties can easily obtain, we can provide theoretical basis for the early diagnosis of cancer by observing the difference of optical properties between normal and cancerous cells. Compared with Mie scattering theory, finite difference time domain (FDTD) algorithm can analyze any complex structure model. In this paper we use mathematical modeling method to establish the single cell mathematical model and with finite difference time domain algorithm to simulate the propagation and scattering of light in the biological cells, you can calculate the scattering of electromagnetic field distribution at anytime and anywhere. With radar cross section (RCS) to measure the results of the scattering characteristics. Due to the difference between normal cells and cancerous cells are embodied in cell shape, size and the refractive index, through the simulation we can get different cell parameters of light scattering information, Find out the cell parameters change the changing rule of the influence on the scattering characteristics and find out change regularity of scattering characteristics. These data can judge very accurate of the cells is normal or cancerous cells.