pH sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging.
EYFP or EGFP improved from GFP in jellyfish are good for such applications. For simultaneous two-color imaging, a
suitable red fluorescent protein is of urgent need. Here a pH sensitive red fluorescent protein, pHuji, is selected and
verified to be compatible with hydrophobic resin embedding and thus may be promising for dual-colour chemical
reactivation imaging in conjunction with EGFP or EYFP.