A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and
characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic
microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes,
adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic
microscope. Quantitative phase images of live cellular specimens are presented.
Evanescent wave phase profiling is incorporated with digital holography into a new surface imaging technique termed
total internal reflection holographic microscopy (TIRHM). Quantitative images of tissue structures and profiles of
cellular membranes are presented to demonstrate the method's performance capabilities. Applications of this technique
include measurements of cellular membranes and their transport processes without the addition of fluorophores. The
angular spectrum method to compensate for tilt anamorphism due to the inclined TIR plane is also discussed.