We propose a new method of flow velocity estimation by analysis of time dependent beating signal using Spectral
Optical Coherence Tomography. The oscillatory beating signal is caused by the Doppler shift of light reflected back
from a mobile object measured in the interferometric set-up. This signal provides information about the velocity of the
movable object. Measurements in model systems prove the method to give accurate results. Additional in vivo
measurements of blood flow in the retinal vessels show potential applicability of this method in the field of biomedical
Novel Fourier domain OCT method and instrumentation using Optical Frequency Comb (OFC) is demonstrated.
This technique can overcome some limitations of Spectral OCT and Optical Frequency domain Imaging (Swept
Source OCT) and enables ultrahigh resolution imaging in 800 nm range. In the novel method external OFC generator
placed after the broad band light source is used. We will demonstrate preliminary data showing the general
performance, advantages and limitations of the Fourier domain OCT method using Optical Frequency Comb
generator based on Fabry-Perot interferometer (F-P). High quality, high resolution cross-sectional images of
biological samples obtained with the presented technique are shown.