Mr. Xiangdong Huang Profile

Xiangdong Huang

at Harbin Institute of Technology

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Proceedings Article | 12 January 2009

Digital pinhole filter used for detection of confocal microscopy images

Xiangdong Huang, Na Liu, Junning Cui

Proc. SPIE. 7133, Fifth International Symposium on Instrumentation Science and Technology

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When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.

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