An approach to image the single virus by using Surface Plasmon Polariton Scattering was presented, which is potential for application in the fast, in-situ virus detection in water environment. Polarized by Surface Plasmon Polaritons, the nanoparaticle emits Surface Plasmon Polariton Scattering and interferes with the incident Surface Plasmon Polaritons, which is easy to be detected. The imaging to Surface Plasmon Polariton excitation was implemented. Meanwhile, the imaging to single 39 nm polystyrene nanoparticle and single T4 phage virus by using the Surface Plasmon Polariton Scattering was obtained. Both the imaging and the accurate counting of single virus can be obtained by using this method.