We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays.
We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of a
specific nucleic acid sequences in a liquid test sample. The capability of distinguishing a one-point mismatch is also
demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route towards portable
biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.