In this study, we performed studies longitudinal sections of long bones of rats by Raman spectroscopy in the simulation reduction of bone mineral density in the animals. The main spectral characteristics of different sections of longitudinal sections of rat humerus are determined. Optical criteria have been introduced to identify bone tissue with reduced mineral density.
The results of application of the Raman spectroscopy method for the estimation of alternative sources for the production of bone spongy implants using the "Lioplast" technology, namely, the femoral heads resected in the operation of hip replacement surgery, are presented. It is shown that Raman spectroscopy can be used to estimate the component composition of the surface of bone implants during their processing. Comparing different sources of sponge bone production, no significant differences were found, but there are differences in the ratio of the intensities of the Raman peaks at wave numbers 1555 cm<sup>-1</sup> and 1665 cm<sup>-1</sup> corresponding to amide II and amide I, and also in the intensity of Raman peaks at wave numbers of 959 cm<sup>-1</sup> (РО<sub>4</sub> <sup>3-</sup> (ν<sub>1</sub>)) and 1068 cm<sup>-1</sup> (СO<sub>3</sub> <sup>2-</sup> (ν<sub>1</sub>) B-type substitution).