Fluorescence fluctuations super-resolution microscopy (FF-SRM) is a powerful tool in imaging and monitoring of biological subcellular structures and dynamics in cells. A variety of image reconstruction algorithms have been developed for FF-SRM. In order to obtain high spatiotemporal resolution, a U-Net-based deep learning method for super-resolution imaging of fluorescence fluctuations was developed. With just 20 frames, super-resolution images reconstructed using U-Net model could be comparable to those reconstructed using VeSRRF algorithm with several hundred frames, demonstrating its capability of advancing imaging capabilities.
Hyperspectral microscopic imaging (HMI) technology is a non-contact optical diagnostic method, which combines hyperspectral imaging (HSI) technology with microscopy to provide both spectral information and image information of the samples to be measured. In this paper, basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and malignant melanoma (MM) were classified based on synthetic RGB image data from HMI cube by using four classification methods extreme learning machine (ELM), support vector machine (SVM), decision tree and random forest (RF). The highest classification accuracy of 0.791±0.060 and a KAPPA value of 0.685±0.095 were obtained when color moment, gray level co-occurrence matrix (GLCM) and local binary pattern (LBP) were used for image feature extraction, feature dimensions were reduced by the PLS, the sample sets were divided by the hold-out method, and the tissues were classified by the SVM model.
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