In this paper, a high-accuracy calibration method for errors resulted from aberration in long focal length measurement, is presented. Generally, Gaussian Equation is used for calculation without consideration of the errors caused by aberration. However, the errors are the key factor affecting the accuracy in the measurement system of a large aperture and long focal length lens. We creatively introduce an effective way to calibrate the errors, with detailed analysis of the long focal length measurement based on divergent light and Talbot interferometry. Aberration errors are simulated by Zemax. Then, we achieve auto-correction with the help of Visual C++ software and the experimental results reveal that the relative accuracy is better than 0.01%.By comparing modified values with experimental results obtained in knife-edge testing measurement, the proposed method is proved to be highly effective and reliable.