Fabrications of free form surface and well array plate demonstrated using glass material by imprint lithography. A metal mold is processed by a Diamond cutting. Using a low Tg glass plate, the mold is pressed to the glass surface and transferred the free form surface. The feature error of the proceed glass surface is within 200nm in 3mm x 6mm field. On the other hand, deep well array for bio-chemical device is transferred on glass surface using ceramics mold. Micro wells with 200 x 200μm in square and 50 μm in depth is achieved without fatal defect using optimized imprint process conditions.