Dr. Yoshihiro Ohta Profile

Dr. Yoshihiro Ohta

at Tokyo Univ of Agriculture and Technology

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 20 September 2011 Paper

Two-photon fluorescence near-field pH measurement for mitochondria activity

Y. Kanazashi, Y. Li, T. Onojima, K. Iwami, Y. Ohta, N. Umeda

Proceedings Volume 8105, 81050E (2011) https://doi.org/10.1117/12.893359

KEYWORDS: Cell death, Luminescence, Near field scanning optical microscopy, Acquisition tracking and pointing, Magnesium, Near field, Calibration, Optical microscopy, Ions, Modulation

Read Abstract +

We proposed a novel pH measurement method based on two-photon fluorescence excitation of a dual-wavelength pHsensitive dye combined with scanning near-field optical microscopy (SNOM) that can be used to evaluate mitochondrial activity. Mitochondria produce ATP using a proton concentration (pH) gradient generated between both sides of their inner membrane. Thus, pH distribution around mitochondria can change with time when mitochondria produce ATP. This pH distribution has attracted interest because of its influence on necrotic cell death. Because ATP depletion causes necrotic cell death, measurement of pH distribution around mitochondria is expected to lead to clarification of the mechanism underlying necrotic cell death. However, it is very difficult to accurately measure pH around mitochondria using conventional pH measurement methods. In this study, a dual-wavelength pH-sensitive dye was excited locally using two-photon fluorescence excitation. In addition, collection-mode SNOM was used to avoid reabsorption by collecting the fluorescent light directly from a florescence point. Using this method, we successfully calibrated pH and observed temporal variations in pH after dropwise addition of acid. Moreover, mitochondrial activity was successfully observed based on these pH changes.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years