Propionibacterium acnes (<i>P. acnes</i>), one of the anaerobic bacterium, causes inflammatory acne. To find a novel medication for treating the inflammation caused by <i>P. acnes</i>, we investigated the anti-bacterial and anti-inflammatory activities of several herbal extracts against <i>P. acnes</i>. The aqueous extracts from five dried herbs, <i>Phellodendron amurense Rupr., Paeonia lactiflora Pallas., Houttuynia cordata Thunb., Agrimonia pilosa Ledeb.</i> and <i>Glycyrrhiza uralensis Fisch.</i>, were prepared and mixed. In this experiment, 1 mg/ml of the herbal extract mixture caused a decrease in the growth of <i>P. acnes</i> and reduced the production of pro-inflammatory cytokines, TNF-α, IL-8, IL-1β and IL-6, in human monocytic THP-1 cells treated with heat-killed <i>P. acnes</i>. Therefore, this herbal extract mixture may possess both anti-bacterial and anti-inflammatory activities against P. acnes and can be a novel therapeutic agent for treating inflammatory acne.