Digital phase microscopy has made great progress in the study of morphology and dynamics of biological cells, especially the homogeneous cells. For the heterogeneous nucleated cells with relatively complex internal structures, there are still few methods of phase unwrapping suitable for observation and measurement. In this paper, a method is proposed to identify the substructure of cells only with their wrapped phases. According to the structural features of real nucleated cells, their similar models are established firstly, and then their wrapped phases are obtained in Cartesian coordinates and polar coordinates. Based on the relationship between the parameters of the two coordinate systems, the pattern recognition and image processing techniques are used to analyze the parcel phase of the single-core and multi-core, regular and irregular kernels, and the central and boundary kernel models. Then, the texture shape distribution, the texture overlap condition, the opening and closing state of the equal phase line, the peak number and the peak position are selected as reference features, and these reference features are combined and optimized as the judgment condition for identifying the nuclear structure model, and the wrapped phase in polar coordinates is screened and identified, which effectively distinguishes three typical types of nuclear cell models. It provides a new, unpacked method for the classification and identification the substructures of the nucleated cells.
Dual-wavelength interferometry is one of the most effective technique in optical metrology and phase imaging by virtue of its unique advantages. In this paper, a fast derivative method for phase extraction is proposed to improve the imaging efficiency in simultaneous dual-wavelength interferometry. In this algorithm, only three off-axis wavelength-multiplexed phase-shifted interferograms are required. And only by calculating the difference between the first interferogram and other interferogram and calculating its first-order derivative, the wrapped phase of each single-wavelength can be obtained. Subsequently, the continuous phase at a synthetic wavelength can be determined freeing from the complex unwrapping procedure. Moreover, the thickness can also be obtained from the phase. Simulation results show that this method is effective and demonstrate its performance of faster computing speed and high accuracy.
Dual-wavelength digital holographic microscopy technique has many important applications in the imaging of biological cells. It has some significant advantages, such as the large measurement range and no phase unwrapping procedures. However, the multi-steps image is required in synchronous interference microscopy, it is not suitable to perform real-time dynamic imaging of samples. In this paper, in order to meet the research and clinical application of dynamic imaging of living cells, a phase recovery algorithm is proposed in dual-wavelength interference and one-step microscopic imaging. This algorithm is on the basis of the principle of the digital holographic off-axis synchronous microscopy image. In this algorithm, only by means of Fourier transform, filtering, frequency-shifting operations, inverse Fourier transform to the dual-wavelength off-axis microscopic interferogram, the continuous phase of the sample can be calculated. Simulation result of the lymphocyte shows that this method is feasible, and the error analysis suggests that it has good accuracy. This approach is free of phase unwrapping, and has no the calculation of the phase shift and no the multi-step imaging. It can recover the phase distribution only from one dual-wavelength interferogram. So it has a high potential application for the technology development, and can be applied to accurate dynamic imaging of biological cells and tissues.
To evaluate the efficacy and safety of photothermal with dinitrophenyl hapten (DNP) for patients with malignant melanoma (MM), Patients with pathology confirmed stage III or IV MM were enrolled. Seventy-two patients were randomized into two groups, DNP alone group (n=36) and DNP plus photothermal therapy group (n=36). The results showed that the patients in the combination treatment group had longer median progression-free survival time (19.0m vs. 12.0m, p=0.007). No severe adverse events were observed in both groups. Thus, the combination of photothermal therapy and DNP maybe a new therapeutic strategy for patients with advanced MM.