Experimental program is designed to analyze the radiation and absorption characteristic of the sky background at near-infrared Oxygen A absorption band of passive ranging based on Oxygen spectral absorption; an acousto-optic tunable hyper spectral imaging spectrometer is used as the measuring device. Under the condition of sunny, cloudy, and snowy weather, the sky background spectral distribution is collected using the acousto-optic tunable hyper spectral imaging spectrometer. Then the Oxygen absorption rate is calculated according to the principle of Oxygen spectrum absorption passive ranging. The measurement result shows: absorption lines exist in the sky background spectral distribution at the Oxygen A absorption band, and the absorption rates are different at different weather conditions. The Oxygen absorption rates are the biggest under snowy weather, bigger under cloudy weather, and the smallest under sunny weather. The general change pattern of Oxygen absorption rate under different weather conditions is obtained and the result has laid solid foundation for suppressing the interference of the background and extracting target spectral accurately in subsequent passive ranging researching.